@article{article_325713, title={Detection of Listeria monocytogenes in Fried Fish, Processing Slab and Tools}, journal={Harran University Journal of the Faculty of Veterinary Medicine}, volume={6}, pages={32–37}, year={2017}, DOI={10.31196/huvfd.325713}, author={Adeshına, İbrahim and Abdulwahab, Mozeedah and Adewale, Yusuf Adetunji and Suleıman, Sa’adu Bala and Tıamıyu, Lateef Oloyede}, keywords={Listeria Monocytogenes, Infection, Prevalence, Fried fish}, abstract={<p class="MsoNoSpacing" style="text-align:justify;"> <span lang="en-us" style="font-size:9pt;" xml:lang="en-us">This study investigated the prevalence
of <i>Listeria monocytogenes </i> in fried
fish, processing slab and tool in Kwara state, Nigeria. <strong> <span style="font-family:Calibri, ’sans-serif’;font-weight:normal;">A total of 2400 fish samples, 800 slabs and
800 tool samples were collected aseptically using stratified sampling methods
for <i>L. monocytogenes </i>analysis <i>. </i>The samples were cultured for <i>L. monocytogenes </i> using PALCAM and fraser
selective agar and supplements. The isolates were characterized using both
biochemical and molecular methods <i>. </i> </span> </strong>The
data obtained were subjected to descriptive <strong> <span style="font-family:Calibri, ’sans-serif’;font-weight:normal;">statistics and </span> </strong> chi-square test, P=0.05. The results have
showed that 528 (22%), 313 (39.13%) and 195 (24.38%) of the fried fish, slab
and tool samples are positive for <i>L.
monocytogenes </i>respectively. Molecular characterization using PCR has shown
that 25.87% of biochemically characterized isolates are identified as <i>L. monocytogenes </i>. <i>Listeria monocyogenes </i>was identified in fried fish (12.88%), slab
(36.41%) and tool (26.41%) samples. There was a significant difference between
the occurrence of <i>L. monocytogenes </i> in
fish, slab and tools. Fried fish, processing slab and tools were heavily
contaminated with <i>L. monocytogenes </i>.
Processing slab and tools were identified to be predisposing factors, and
therefore calls for attentions. </span> </p>}, number={1}, publisher={Harran University}