TY - JOUR T1 - Salvadora Persica (Misvak) Bitkisinden Elde Edilen Uçucu Yağın Antioksidan ve Antimikrobiyal Aktivitelerinin Araştırılması TT - Investigation of antioxidant and antimicrobial activity of essential oil from Salvadora Persica (miswak) AU - Apaydın, Elif PY - 2018 DA - June Y2 - 2018 DO - 10.16984/saufenbilder.332517 JF - Sakarya University Journal of Science JO - SAUJS PB - Sakarya University WT - DergiPark SN - 2147-835X SP - 1001 EP - 1006 VL - 22 IS - 3 LA - tr AB - Bu çalışmanınamacı; Salvadora persica (misvak)bitkisinden elde edilen uçucu yağınantioksidan ve antimikrobiyal etkilerini araştırmaktır. Bu bitkininçalışılma sebebi; misvağın uzun zamandır tüm dünyada diş sağlığı ve temizliğialanında kullanılmasıdır. Bu çalışma farklı spektroskobik yöntemler ve GC-MSile açıklamaya çalışıldı. Bu amaçla 250 gram misvak tozu,hidrodistilasyon yöntemi ile clevenger aparatında yağ ekstraksiyonu yapıldı,susuz Na2SO4ile su uzaklaştırıldı ve 4 ͦ C buzdolabında yağlar saklandı. Uçucu yağbileşenleri GC-MS ile tanımlandı. Uçucu yağların antimikrobiyal özellikleridisk difüzyon yöntemi ile Parvathy vearkadaşları metoduna göre yapıldı. DPPH radikal süpürme ve Hidrojen peroksitsüpürme kapasitesi spetkrofotometrik yöntem ile yapıldı. Farklı mineraller veağır metalleri ICP-MS ile analiz edildi. KW - Salvadora persica KW - uçucu bileşen KW - antioksidan aktivite KW - antimikrobiyal aktivite N2 - The purpose of this study thatinvestigation antioxidant and antimicrobial activity by various methods ofessential oil Salvadora persica (miswak) because the choice of this plant thathave used for long time to improve dental health and to get oral hygiene,throughout the World. It was tried to be explained as follows GC-MS (gaschromatography mass spectrometry) and spectrophotometric individually and incombination. For this purpose; the essential oils of miswak (250 g) wereobtained by hydrodistillation at 100 °C with a clevenger-type apparatus. Theessential oils obtained were separated from water and dried over anhydrous Na2SO4,and stored at 4 °C. The components of the essential oil were identified byGC-MS analyses. The antimicrobial assay of the essential oils was tested by thepour plate method according to the reports of Parvathy et al. DPPH(1,1-diphenyl-2-picrylhydrazyl) radicals scavenging assay and superoxide anionradical scavenging activity were measured spectrophotometric method. The differencemineral and heavy metals would explain with ICP-MS (Inductively Coupled Plasma-MassSpectrometer). CR - [1] ROTIMI, V.O. and MOSADOMI, H.A., The Effect of Crude Extracts of nine African Chewing Sticks on Oral Anaerobes, Journal of Medicinal Microbiology, 1987; 23, 55–60. CR - [2] ODEBIYI O.O. and E.A. SOFOWARA E.A., Antimicrobial Alkaloids from a Nigerian Chewing Stick (Fagara zanthoxyloides), Planta Medica, 1979; 36: (7), 204–207. CR - [3] MOUSTAFA, M.H., and SADEK S.I., The Efficiency of Miswak Concantrate Toothpaste , I Clinical Report , Egyptain Dentist Journal, 1987; 33: 75-84. CR - [4] W.H.O., Prevention of Oral Diseases. Geneva, 1987. CR - [5] WHO., Concensus Statement on Oral Hygiene, Int Dentist Journal. 2000;50, 139. CR - [6] FAROOQUI, M.I.H. and SRIVASTAVA, J.G. The tooth brush tree, Quarterly Journal of Crude Drug Research, 1968; 8:1297-1299. CR - [7] EZMIRLY, S.T., EL-NASR, M.S., Isolation of Glucotropaeolin from Salvadora Persica L. Chemical Society Pakistan, 1981;3:9-12. CR - [8] WOLINSKY, L.E. and SOTE, E.O., Isolation of Natural Plaque-Inhibiting Substances from ‘Nigerian Chewing Sticks’, Caries Research, 1984; 18, 216–225. CR - [9] CHEN, D.N., DOGAN, A. V. and DOGAN, M. M., Journal Elec.Micro.Tech., 1987;5, 145 CR - [10] HATTAB F.N., Meswak: the Natural Toothbrush, Journal Clinical Dent. 1997;8:125–129. CR - [11] JANSEN, A.M., SCHAFFER, J.C. and BERGHEİM-SVENDSEN, A., Antimicrobial Activity of Essential Oils: a 1976-1986 Literature Review. Aspects of the test methods, Planta Medica, 1987;53:(5) 395–408. CR - [12] El-TAWIL B.H., Chemical Constituents of Indigenous Plants Used in Native Medicine of Suidi Arabia, II. Arab Gulf Journal Scient Res., 1983; 1:395–419. CR - [13] E.M. Gallati, A.M. Forestieri, M.T. Monforted’Aquino and A. Rossitto (1997). Second International Symposium on Natural Drugs, Abstracts, Maratea, September 28–October 1. CR - [14] BLOIS M. S., Antioxidant determinations by the use of a stable free radical, Nature, 1958; 181: 1199–1200. CR - [15] RUCH R. J., CHENG S. J., KLAUNIG J. E., “Prevention of cytotoxicity and inhibition of intracellular comunication by antioxidant catechins isolated from Chinese grean tea.” Carcinogenesis, 1989 ;10 (6), 1003-1008. CR - [16] CHU, S.S., LIU, Q.R., LIU, Z.L., Insecticidal Activity and Chemical Composition of the Essential oil of Artemisia Vestita from China Against Sitophilus Zeamais, Biochemical Systematics and Ecology, 2010; 38: 489–492. CR - [17] ŠARIŠ, C. L., ŢABARKAPA, S. I., BELJKAŠ, M. B., MIŠAN, C. A., SAKAŢ, B. M., PLAVŠIŠ, V.D., Antimicrobial Activity of Plant Extracts from Serbia. Food Processing, Quality and Safety, 2009; 1(2): 1-5. UR - https://doi.org/10.16984/saufenbilder.332517 L1 - https://dergipark.org.tr/en/download/article-file/462731 ER -