TY - JOUR T1 - Effect of Hardaliye on FoxM1 Gene Expression Level of HT-29, DU-145, HeLa Cancer Cells and CF-1 (Mouse Embryonic Fibroblast) TT - Hardaliyenin HT-29, DU-145, HeLa Kanser Hücreleri ve CF-1 (Fare Embriyonik Fibroblast) Hücresi FoxM1 Gen Expresyon Seviyelerine Etkisi AU - Kahraman Ilıkkan, Özge PY - 2017 DA - August DO - 10.24323/akademik-gida.333633 JF - Akademik Gıda JO - Akademik Gıda PB - Sidas Medya Ltd. Şti. WT - DergiPark SN - 1304-7582 SP - 119 EP - 123 VL - 15 IS - 2 LA - en AB - Chemotherapeutic agents may influence both cancer cells and healthycells, therefore alternative therapeutic agents or targets have become a milestone of cancer cure. Forkhead box M1 (FoxM1)is a transcriptional regulator and a novel cancer therapy target asoverexpressed in cancer cells, and its inhibitors are considered as potentialtherapeutic agents to halt cancer progression. Therefore, in this study, theeffect of “Hardaliye” on cancer cells anda healthy cell line was primarily investigatedby MTT (3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide) and lateron, FoxM1 gene expression levels were determined. Hardaliye, especially when diluted ten and twenty-fold, decreasedviability percentage of all cancer cells and did not affect healthy cells.FoxM1 levels of cancer cells drastically decreased especially in HT-29 cellswhile did not statistically change their levels in the healthy cell line. KW - FoxM1 KW - qRT-PCR KW - Cancer cell lines KW - CF-1 KW - Gene expression N2 - Kemoterapötik ajanlar hem kanser hücrelerini hem de sağlıklı hücreleri etkilemektedirbu nedenle alternatif terapötik ajanlar veya hedefler kanser tedavisininkilometre taşı olmuştur. Forkhead box M1 (FoxM1) transkripsiyonel regülatördürve kanser hücrelerinde daha fazla eksprese olması nedeniyle yeni kanser terapihedefi olmuştur. Fox M1 inhibitörleri, kanser gelişimini engelleyen potansiyel terapötikajanlardır. Bu nedenle, çalışmada, Hardaliye’nin kanser hücreleri ve sağlıklıhücre hattı üzerine etkisi MTT yöntemiyle öncelikle belirlenmiş ve sonrasında,FoxM1 gen ekspresyon seviyeleri tayin edilmiştir. Hardaliye, özellikle on veyirmi-kat seyreltildiğinde bütün kanser hücrelerinin yüzde canlılığını düşürmüşancak sağlıklı hücreleri etkilememiştir. FoxM1 seviyeleri sağlıklı hücrehatlarında istatistiksel olarak değişmezken, kanser hücrelerinde özellikleHT-29 hücrelerinde epeyce düşmüştür. CR - [1] Wang, Z., Ahmad, A., Li, Y., Banerjee, S., Kong, D., Sarkar, F.H., 2010. Forkhead box M1 transcription factor: a novel target for cancer therapy. Cancer Treatment Reviews 36(2): 151-156. CR - [2] Laoukili, J., Stahl, M., Medema, R.H., 2007. FoxM1: at the crossroads of ageing and cancer. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer 1775(1): 92-102. CR - [3] Koo, C.Y., Muir, K.W., Lam, E.W.F., 2012. 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FoxM1, a critical regulator of oxidative stress during oncogenesis. The EMBO Journal 28(19): 2908-2912. UR - https://doi.org/10.24323/akademik-gida.333633 L1 - https://dergipark.org.tr/en/download/article-file/333943 ER -