TY - JOUR TT - Grafen Oksit-Polianilin Nanokompozit Temelli Amperometrik Glukoz Biyosensörü Geliştirilmesi AU - Çoğal, Sadık PY - 2017 DA - August DO - 10.24323/akademik-gida.333663 JF - Akademik Gıda JO - Akademik Gıda PB - Sidas Medya Ltd. Şti. WT - DergiPark SN - 1304-7582 SP - 124 EP - 129 VL - 15 IS - 2 KW - Glucose KW - Glucose oxidase KW - Graphene oxide KW - Polyaniline KW - Biosensor N2 - Bu çalışmada, Pt elektrot, grafenoksit-polianilin (GO-PANI) nanokompoziti ile modifiye edilerek amperometrikglukoz biyosensörü geliştirilmesinde kullanılmıştır. Modifiye edilmiş Ptelektroda glukoz oksidaz (GOD) enzimi çapraz bağlayıcı kullanılarak immobilizeedilmiştir. Bu şekilde hazırlanan Pt/GO-PANI/GOD biyosensörünün optimum çalışmakoşullarında amperometrik yöntemle glukoza karşı cevabı çalışılmış ve 36.11 μA mM-1 cm-2 hassasiyet, 0.59-9.15 mM lineer çalışma aralığı ve 0.24 mM tayin limiti sergilemiştir. Geliştirilenglukoz biyosensörü üzerine askorbik asit (AA) ve ürik asidin (UA) girişimetkileri incelenmiş ve biyosensörün glukoza karşı oldukça seçici olduğugözlenmiştir. CR - [1] Mello, L.D., Kubota, L.T., 2002. Review of the use of biosensors as analytical tools in the food and drink industries. Food Chemistry 77: 237-256. CR - [2] Borisov, S.M., Wolfbeis, O.S., 2008. Optical Biosensors. Chemical Reviews 108: 423-461. CR - [3] Luong, J.H.T., Bouvrette, P., Male, K.B., 1997. Developments and applications of biosensors in food analysis. Trends in Biotechnology 15: 369-377. CR - [4] McGrath, T.F., Elliott, C.T., Fodey, T.L., 2012. Biosensors for the analysis of microbiological and chemical contaminants in food. Analytical and Bioanalytical Chemistry 403: 75-92. CR - [5] Ayenimo, J.G., Adeloju, S.B. 2017. Amperometric detection of glucose in fruit juices with polypyrrole-based biosensor with an integrated permselective layer for exclusion of interferences. Food Chemistry 229: 127-135. CR - [6] Boujtita, M., Boitard, M., El Murr, N., 1999. Development of renewable surface biosensors to meet industrial needs for measurement of glucose in fruit juices. Biosensors and Bioelectronics 14: 545-553. CR - [7] Ocvirk, G., Buck, H., DuVall, S.H., 2017. Electrochemical glucose biosensors for diabetes care. Bioanalytical Reviews 6. CR - [8] Marquette, C.A., Degiuli, A., Blum, L.J., 2003. Electrochemiluminescent biosensors array for the concomitant detection of choline, glucose, glutamate, lactate, lysine and urate. Biosensors and Bioelectronics 19: 433-439. CR - [9] Wang, J., 2008. Electrochemical glucose biosensors. Chemical Reviews 108: 814-825. CR - [10] Hong, S.G., Kim, J.H., Kim, R.E., Kwon, S.J., Kim, D.W., Jung, H.T., Dordick, J.S., Kim, J., 2016. Immobilization of glucose oxidase on graphene oxide for highly sensitive biosensors. Biotechnology and Bioprocess Engineering 21: 573-579. CR - [11] Ekanayake, E.M.I.M., Preethichandra, D.M.G., Kaneto, K., 2008. Effect of glucose oxidase immobilizing techniques on performances of nano scale polypyrrole glucose biosensors. Japanese Journal of Applied Physics 47: 1321-1324. CR - [12] Schuhmann, W., Huber, J., Mirlach, A., Daub, J., 1993. Covalent binding of glucose oxidase to functionalized polyazulenes. The first application of polyazulenes in amperometric biosensors. Advanced Materials 5: 124-126. CR - [13] Stejskal, J., Gilbert, R.G., 2002. Polyaniline. Preparation of a conducting polymer (IUPAC Technical Report). Pure Applied Chemistry 74: 857–867. CR - [14] Geim, A.K., 2009. Graphene: status and prospects. Science 324: 1530–1534. CR - [15] Chen, D., Feng, H., Li, J., 2012. Graphene oxide: Preparation, functionalization, and electrochemical applications. Chemical Reviews 112: 6027-6053. CR - [16] Xu, G., Wang, N., Wei, J., Lv, L., Zhang, J., Chen, Z., Xu, Q., 2012. Preparation of graphene oxide/polyaniline nanocomposite with assistance of supercritical carbon dioxide for supercapacitor electrodes. Industrial and Engineering Chemistry Research 51: 14390-14398. CR - [17] Yang, Y., Kang, M., Fang, S., Wang, M., He, L., Zhao, J., Zhang, H., Zhang, Z., 2015. Electrochemical biosensor based on three-dimensional reduced graphene oxide and polyaniline nanocomposite for selective detection of mercury ions. Sensors and Actuators, B: Chemical 214: 63-69. CR - [18] Hummers, W.S., Offeman, R.E., 1958. Preparation of Graphitic Oxide. Journal of the American Chemical Society 80: 1339-1339. CR - [19] Gui, D., Liu, C., Chen, F., Liu, J., 2014. Preparation of polyaniline/graphene oxide nanocomposites for the application of supercapacitor. Applied Surface Science 307: 172-177. CR - [20] Kamin, R.A., Wilson, G.S., 1980. Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer. Analytical Chemistry 52: 1198-1205. CR - [21] Rogers, M.J., Brandt, K.G., 1971. Interaction of d-glucal with Aspergillus niger glucose oxidase. Biochemistry 10: 4624–4630. CR - [22] Feng, X., Cheng, H., Pan, Y., Zheng, H., 2015. Development of glucose biosensors based on nanostructured graphene-conducting polyaniline composite. Biosensors and Bioelectronics 70: 411-417. CR - [23] Kong, F.Y., Gu, S.X., Li, W.W., Chen, T.T., Xu, Q., Wang, W., 2014. A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode: Toward whole blood glucose determination. Biosensors and Bioelectronics 56: 77-82. CR - [24] Hansen, B., Hocevar, M.A., Ferreira, C.A., 2016. A facile and simple polyaniline-poly(ethylene oxide) based glucose biosensor. Synthetic Metals 222: 224-231. CR - [25] Unnikrishnan, B., Palanisamy, S., Chen, S.M., 2013. A simple electrochemical approach to fabricate a glucose biosensor based on graphene-glucose oxidase biocomposite. Biosensors and Bioelectronics 39(1): 70-75. CR - [26] Çoğal, S., Şen Gürsoy, S., Çelik Çoğal, G., Gürsoy, O., 2016. Sütte laktoz tayini için biyosensörlerin kullanımı. Akademik Gıda 14(1): 33-42. UR - https://doi.org/10.24323/akademik-gida.333663 L1 - https://dergipark.org.tr/en/download/article-file/333944 ER -