@article{article_334792, title={FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith)}, journal={Trakya University Journal of Natural Sciences}, volume={19}, pages={63–69}, year={2018}, DOI={10.23902/trkjnat.334792}, url={https://izlik.org/JA52FX67EU}, author={Yörük, Emre and Sefer, Özlem}, keywords={Fusarium culmorum,PCR,genetic characterization,genetic diversity,linear growth rate}, abstract={<p> <i> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">Fusarium culmorum </span> </i> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb"> (W.G. Smith) leads
to </span> <span style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;">economic </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb"> losses in wheat and
barley fields in Turkey as well as in many countries worldwide as a result of
head blight and crown rot diseases. In this study, <i>in vitro </i>growth capacity of 33 <i>F.
culmorum </i> isolates originating from Turkey and the relationship between phenotypic
and genetic characteristics obtained based on similarities of <i>FcMgv1 </i>, <i>FcStuA </i> and <i>FcVeA </i>genes
were </span> <span style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;">investigated </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">. Linear growth rate
values were recorded at 4 <sup>th </sup> and 7 <sup>th </sup> days of incubation.
The mean linear growth rate values ranged from <a>7.58±1.06
to 14.7±1.26 </a>mm/day. The isolates F2 and 18F with relatively high linear
growth values and the isolates 12F and F19 with relatively low linear growth
values, were selected to be used in multiloci based genotyping analysis. <i>FcMgv1 </i>, <i>FcStuA </i> and <i>FcVeA </i> genes
were amplified in lengths of 1733, 2001 and 1898bp, respectively. The genes
were sequenced, aligned and then subjected to BLASTn and to maximum likelihood
topology analysis. Nucleotide sequence of each gene showed maximum hit with
associated genes deposited in NCBI with 0.0-0.0 E-values and 1188 to 3256 bit
scores. Alignment analysis resulted in at least 89% bootstrap support.
Moreover, isolates with similar linear growth rates were co-clustered in
phylogenetic analysis. The findings obtained in this study showed that the
three genes which are essential for fungal survival could be used in genetic
characterization analysis and in revealing the associations between their
genetic and phenotypic characteristics. </span> <br /> </p>}, number={1}