TY  - JOUR
TT  - Polymorphism in Exon 7 of β- Lactoglobulin (β-LG) Gene and Its Association with Milk Yield in Saanen Goats
AU  - Işık, Raziye
PY  - 2017
DA  - September
JF  - Tekirdağ Ziraat Fakültesi Dergisi
JO  - JOTAF
PB  - Tekirdag Namik Kemal University
WT  - DergiPark
SN  - 1302-7050
SP  - 35
EP  - 40
KW  - β-LG
KW  - PCR-RFLP
KW  - Saanen
KW  - SNP
KW  - SacII
N2  - β-Lactoglobulin (β-LG) is one of milk protein and has important function on technological properties of milk such ascheese making. The relations between whey protein genes and milk yield/ composition have been investigated inprevious researches. β-LG can be utilized as a candidate gene for selection and breeding programs to increase milkyield and protein quality. The aim of this study is to investigate the β-LG gene polymorphism and relation between β-LG genotypes and milk yield. In this study, a total of 74 purebred Saanen goats originated from Australia were usedto detect polymorphism with PCR-RFLP method. SacII digestion in 427 bp of β-LG exon 7 (GenBank: Z33881.1) wasrevealed. Two alleles (S1, S2) and 3 genotypes (S1S1, S1S2, S2S2) were determined in β-LG/ SacII locus. The β-LG/S1S1genotype with only one restriction site revealed two bands (347 bp and 80 bp). The β-LG/S1S2 heterozygote genotypegenerated three bands (427 bp, 347 bp and 80 bp). An undigested product, 427 bp was β-LG/S2S2 genotype whichwas due to a single nucleotide substitution at position g.4601G>A. S1S2 with genotype frequency (43.3%) higher thanthe other genotypes. S1 allele frequency was determined predominantly. Deviation from Hardy-Weinberg equilibriumwas not identified in the Saanen breed. In β-LG/ SacII locus, a significant relationship was not found betweengenotypes and lactation milk yield. However, β-LG/ S1S1 genotype was observed to have higher lactation milk yield. Itis concluded that β-LG gene could be used as a molecular marker for economic traits such as milk yield andcomposition.
CR  - Ağaoğlu, Ö.K., B.Ç. Kul, B. Akyüz, Ö. Elmaz, M. Ö. Metin, M. Saatçı and O. Ertuğrul, 2012.Identification of β- Lactoglobulin gene SacII polymorfphism in Honamlı, Hair and Saanen goat breeds reared in Burdur vicinity. Kafkas
Univ Vet Fak Derg. 18 (3) 385-388
UR  - https://dergipark.org.tr/en/pub/jotaf/issue//336406
L1  - https://dergipark.org.tr/en/download/article-file/338199
ER  -