@article{article_337640, title={In vitro Inhibition Effects of Some Metal Ions on Glutathione Reductase Purified from Capoeta trutta Kidney}, journal={Aquaculture Studies}, volume={17}, pages={385–394}, year={2017}, DOI={10.17693/yunusae.v17i31121.337640}, author={Kırıcı, Muammer and Kırıcı, Mahinur and Atamanalp, Muhammed}, keywords={Glutathione reductase,Capoeta trutta,kidney,inhibitor,purification}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:150%;"> <span style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;">Glutathione reductase (EC: 1.8.1.7; GR)
was purified from the kidney of the teleost fish <i>Capoeta trutta </i>. The purification procedure consisted of three
steps: preparation of homogenate, ammonium sulfate fractionation and affinity
chromatography on 2’,5’-ADP Sepharose 4B. The enzyme was purified 794-fold with
a yield of 35.4% and a specific activity of 11.91 U/mg proteins. In order to
control enzyme purity, SDS-PAGE was done and showed a single band for enzyme. A
single band was obtained approximately at 55 kDa. In addition, inhibitory
effects of metal ions (Ag <sup>+ </sup>, Co <sup>+2 </sup>, Ni <sup>+2 </sup>, Cu <sup>+2 </sup>
and Zn <sup>+2 </sup>) on fish kidney glutathione reductase were investigated. K <sub>i </sub>
constants and IC <sub>50 </sub> values for metal ions were determined by
Lineweaver–Burk graphs and plotting activity % vs. [I], respectively. IC <sub>50 </sub>
values were 0.00078, 0.622, 0.722, 0.073 and 0.519 mM, and K <sub>i </sub>
constants were 0.000394 ± 0.0002, 0.235 ± 0.027, 0.279 ± 0.048, 0.026 ± 0.008
and 0.382 ± 0.024 mM for Ag <sup>+ </sup>, Co <sup>+2 </sup>, Ni <sup>+2 </sup>, Cu <sup>+2 </sup>
and Zn <sup>+2 </sup>, respectively. From these results, we showed that Ag <sup>+ </sup>
is the most potent inhibitor of glutathione reductase enzyme. </span> </p> <p> </p>}, number={4}, publisher={Su Ürünleri Merkez Araştırma Enstitüsü}