TY - JOUR TT - Kolorimetrik MTT testi kullanarak geleneksel protez kaide materyali ile yumuşak astar materyalinin in vitro sitotoksik özelliklerinin değerlendirilmesi AU - Akay, Canan AU - Çakırbay Tanış, Merve PY - 2018 DA - June Y2 - 2017 DO - 10.17343/sdutfd.337797 JF - Medical Journal of Süleyman Demirel University JO - Med J SDU PB - Süleyman Demirel University WT - DergiPark SN - 1300-7416 SP - 157 EP - 166 VL - 25 IS - 2 KW - Conventional denture base material KW - soft liners KW - cytotoxicity KW - thermal cycles N2 - ÖzetAmaç:Bu çalışmada protez yapımında kullanılan geleneksel kaide materyali ile yumuşakastar materyalinin fare fibroblast hücreleri üzerinde zamanla meydana gelensitotoksik etkilerinin değerlendirilmesi amaçlandı.Materyalve Metod: Protez kaide materyali (rodeks) ve yumuşak astar materyalinin (dentusil)disk şekilli test numuneleri üreticinin talimatlarına göre aseptik şartlaraltında hazırlandı.. Örnekler, ağız ortamını taklit etmek için 5.000 termaldöngüye tabi tutuldu. Yaşlanma prosedürlerini takiben, materyallerin sitotoksiketkisi, 24 saat, 48 saat ve 72 saatlik hücre inkubasyon döneminden sonra L929fare fibroblast hücreleri kullanılarak [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide] testi ile değerlendirildi. Her grup için hücre canlılığı değerlerihesaplandı. Verilerin istatistiksel analizi, iki yönlü tekrarlanan bir ölçümyöntemi kullanılarak gerçekleştirildi. (P <0.001)Bulgular: 24 saat ve 48 saatinkubasyon periyodunda yumuşak astar materyali, 72 saat inkübasyon periyodundaise kaide materyali daha fazla hücre canlılığı göstermiştir. İstatistikselolarak iki materyal arasında anlamlı fark bulunmuştur. İnkübasyon periyotları arasındaise 24 saat inkübe edilen grup 48 saat ve 72 saatden istatistiksel olarakfarklıdır. 72 saat ve 48 saat arasında anlamlı fark bulunamamıştır.Sonuç: Kaide materyallerininaltına kullanmış olduğumuz yumuşak astar materyali kaide materyaline göre dahabiyouyumludur. CR - 1. Song YH, Song H J, Han MK, Yang HS, Park YJ. Cytotoxicity of soft denture lining materials depending on their component types. Int J Prosthodont, 2014;27(3):229-35 CR - 2. Gonçalves TS, Morganti MA, Campos LC, Rizzatto SM, Menezes LM. Allergy to autopolymerized acrylic resin in an orthodontic patient. Am J Orthod Dentofacial Orthop 2006;129:431-5 CR - 3. Chaves CDAL, Machado AL, Vergani CE, de Souza RF, Giampaolo ET. Cytotoxicity of denture base and hard chairside reline materials: a systematic review. J Prosthet Dent, 2012:107(2); 114-127 CR - 4. Karakış D, Akay C, Erdönmez D, Doğan A. Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi. 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