TY - JOUR TT - Production of Lipase and Protease Enzymes from Streptomyces sp. K22 and K30 Strains AU - Özcan, Kadriye AU - Çorbacı, Cengiz PY - 2017 DA - December DO - 10.31466/kfbd.343293 JF - Karadeniz Fen Bilimleri Dergisi JO - KFBD PB - Giresun University WT - DergiPark SN - 2564-7377 SP - 128 EP - 135 VL - 7 IS - 2 KW - Lipaz KW - proteaz KW - stabilite KW - Streptomyces sp. N2 - Microbial enzymes are important biotechnologicaltools. They play role in several biological and chemical reactions and haveacquired a significant place in different fields in various industries. Amongmicrobial enzymes, lipases and proteases are important members of the group.Therefore, the selection of microorganisms producing these enzymes and thedetermination of their characteristics trigger researchers to work on thisfield. In this study, it was aimed to investigate production capacities ofextracellular lipase and protease enzymes by actinobacteria strains isolatedfrom Black Sea sediment samples and to determine their pH and temperaturestabilities. In lights of the results obtained from the study, it was found thatthese organisms are well-producer of lipase and protease enzymes, and theirenzymes have a wide range of pH and temperature stability. CR - Aehle, W., (2007). Enzymes in Food Applications, Enzymes In Industry – Production and Applications. Wiley-Verlag. Bradford, M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254. CR - Ferrero, M.A., Castro, G.R., Abate, C.M., Baigori, M.D., Sineriz, F., (1996). Thermostable alkaline proteases of Bacillus licheniformis MIR 29: Isolation, production and characterization. Applied Microbiology and Biotechnology, 45, 327. CR - Garcia-Sanchez, A., Cerrato, R., Larrasa, J., Ambrose, N.C., Parra, A., Alonso, J.M., Hermoso-de-Mendoza, M., Rey, J.M., Mine, M.O., Carnegie, P.R., Ellis, T.M., Masters, A.M., Pemberton, A.D., Hermoso-de-Mendoza, J., (2004). Characterisation of an extracellualr serine protease gene (nasp gene) from Dermatophilus congolensis. FEMS Microbiology Letters, 231: 53-57. CR - Ghorbel, S., Kammoun, M., Soltana, H., Nasri, M., Hmidet, N., (2014). Streptomyces flavogriseus HS1: Isolation and characterization of extracellular proteases and their compatibility with laundry detergents. BioMed Research International, 2014, 1-8. CR - Godtfredsen, S.E., (1990). Microbial lipases. In W.M. Fogarty and E.T. Kelly (Eds.), Microbial Enzymes and Biotechnology (pp. 255-274). Elsevier: Amsterdam. Goodfellow, M., Williams, S.T., (1983). Ecology of actinomycetes. Annual Review of Microbiology, 37, 189-216. CR - Izrael-Zivkovic, L., Gojgic-Cvijovic, G., Karadzic, I., (2010). Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. Journal of the Serbian Chemical Society, 75(8), 1041-1052. CR - Kumar, C.G., Takagi, H., (1999). Microbial alkaline protease; from a bio industrial view point. Biotechnology Advances, 17, 561-594. CR - Kumar, S., Kikon, K., Upadhyay, A., Kanwar, S.S., Gupta, R., (2005). Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification, 41, 38-44. CR - Maal, K.B., Emtiazi, G., Nahvi, I., (2009). Production of alkaline protease by Bacillus cereus and Bacillus polymixa in new industrial culture mediums and its immobilization. African Journal of Microbiology Research, 3, 491. CR - Mehrotra, S., Pandey, P.K., Gaur, R., Darmwal, N.S., (1999). The production of alkaline protease by a Bacillus species isolate. Bioresource Technology, 67, 201-203. CR - Mobarak-Qamsari, E., Kasra-Kermanshahi, R., Moosavi-Nejad, Z., (2011). Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110. Iranian Journal of Microbiology, 3(2), 92-98. CR - Oberoi, R., Beg, Q.K., Puri, S., Saxena, R.K., Gupta, R., (2001). Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World Journal of Microbiology&Biotechnology, 17, 493-497. CR - Sangeetha, R., Geetha, A., Arulpandi, I., (2008). Optimization of protease and lipase production by Bacillus pumilus SG 2 isolated from an industrial effluent. The Internet Journal of Microbiology, 5(2), 1-8. CR - Rapp, P., Backhaus, S., (1992). Formation of extracellular lipase by filamentous fungi, yeasts and bacteria. Enzyme and Microbial Technology, 14, 938-943. CR - Rathi, P., Saxena, R.K., Gupta, R., (2001). A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochemistry, 37, 187-192. CR - Remya, M., Vijayakumar, R., (2008). Isolation and characterization of marine antagonistic actinomycetes from west coast of India. Medicine and Biology, 15(1), 13-19. CR - Sztajer, H., Maliszewska, I., Wieczorek, J., (1988). Production of exogenous lipase by bacteria, fungi and actinomycetes. Enzyme and Microbial Technology, 10, 492-497. CR - Ülker, S., Özel, A., Çolak, A., Alpay Karaoğlu, Ş., (2011). Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil. Turkish Journal of Biology, 35, 543-550. CR - Wiseman, A., (1987). Handbook of Enzymes Biotechnology, Second Edition. Ellis Horwood Limited: Chichester. UR - https://doi.org/10.31466/kfbd.343293 L1 - https://dergipark.org.tr/en/download/article-file/381139 ER -