TY - JOUR T1 - OPTIMIZATION OF ULTRASONIC-ASSISTED EXTRACTION PARAMETERS FOR ANTIOXIDANTS FROM Curcuma longa L. TT - OPTIMIZATION OF ULTRASONIC-ASSISTED EXTRACTION PARAMETERS FOR ANTIOXIDANTS FROM Curcuma longa L. AU - Şahin, Saliha PY - 2018 DA - October Y2 - 2018 DO - 10.23902/trkjnat.344985 JF - Trakya University Journal of Natural Sciences JO - Trakya Univ J Nat Sci PB - Trakya University WT - DergiPark SN - 2528-9691 SP - 121 EP - 128 VL - 19 IS - 2 LA - en AB - In this study, ultrasonication wasused to extract antioxidant compounds such as polyphenols, flavonoids, andcurcuminoids from turmeric (Curcuma longaL.) The influences of time, ethanol concentration and temperature as three independentfactors on the extraction of the total phenolic content were evaluated by theFolin-Ciocalteu method and the antioxidant capacities by the ABTS and chromiumreducing antioxidant capacity (CHROMAC) methods. The central composite design(CCD) with a multi-response surface methodology (MRSM) was used for thestatistical modeling of the response data followed by the regression and analysis of variance(ANOVA) to determine the significance of the model and the factors. Theresponse predictions obtained at optimum extraction conditions of an extractiontime of 64min, an ethanol concentration of 82% (v/v) and an extractiontemperature of 32°C were 47.32mg GAE/g (for Folin-Ciocalteu), 29.15 (for ABTS)and 5.17mg TE/g (for CHROMAC). The predicted values obtained from themulti-response surface methodology agreed with the experimental values data 95%confidence level. These data indicate that the multi-response surfacemethodology is applicable for optimizing the ultrasonic-assisted extraction ofantioxidant compounds from C. longa. KW - Curcuma longa L. KW - ultrasonic-assisted extraction KW - antioxidant capacity KW - phenolic compounds KW - multi-response surface methodology N2 - Buçalışmada ultrasonikasyon, Curcuma longa L.’dabulunan polifenol, flavonoid ve kurkuminoid gibi antioksidan bileşiklerinekstraksiyonu için kullanılmıştır. Zaman, etanol derişimi ve sıcaklık gibi 3bağımsız değişkenin toplam fenolik madde (Folin yöntemi) ve antioksidankapasite (ABTS ve CHROMAC yöntemi) üzerine etkisi değerlendirilmiştir. Merkezikompozit dizayn ile çok yanıtlı yüzey analiz yöntemi, sonuçların istatistikselmodellenmesi, model ve faktörler arası etkileşimi belirlemek için regresyon veANOVA analizinde kullanılmıştır. Belirlenen optimum koşullarda (64dk ekstraksiyonzamanı, %82 (v/v) etanol derişimi ve 32°C ekstraksiyon sıcaklığı)toplam fenolik madde miktarı 47,32mg GAE/g, antioksidan kapasite 29,15 (ABTS)ve 5,17 (for CHROMAC) mg TE/g bulunmuştur. Bu deneysel değerler ile tahminideğerler %95 güven aralığında birbiri ile uyumludur. Buna göre çok yanıtlıyüzey analiz tekniği (MRSM), C. longa’danantioksidan bileşiklerin ultrasonik-destekli ekstraksiyon optimizasyonu içingüvenle kullanılabilir. CR - 1. Aybastıer, Ö. & Demir, C. 2010. Optimization of immobilization conditions of Thermomyces lanuginosus lipase on styrene-divinylbenzene copolymer using response surface methodology. Journal of Molecular Catalysis B: Enzymatic, 63: 170-178. CR - 2. Baş, D. & Boyaci, I.H. 2007. Modeling and optimization I: Usability of response surface methodology. Journal of Food Engineering, 78: 836-845. CR - 3. Dailey, A. & Vuong, Q.V. 2015. Optimization of aqueous extraction conditions for recovery of phenolic content and antioxidant properties from macadamia (Macadamia tetraphylla) skin waste. Antioxidants, 4: 699-718. CR - 4. Dorta, E., Lobo, M.G. & González, M. 2012. Using drying treatments to stabilise mango peeland seed: effect on antioxidant activity. LWT-Food Science and Technology, 45: 261-268. CR - 5. Işık, E., Şahin, S. & Demir, C. 2013. Development of a new chromium reducing antioxidant capacity (CHROMAC) assay for plants and fruits. Talanta, 111: 119-124. CR - 6. Kant, V., Gopal, A., Pathak, N.N., Kumar, P., Tandan, S.K. & Kumar, D. 2014. Antioxidant and anti-inflammatory potential of curcum in accelerated the cutaneous woundhealing in streptozotocin-induced diabeticrats. International Immunopharmacology, 20: 322-330. CR - 7. Kiamahalleh, M.V., Najafpour-Darzi, G., Rahimnejad, M., Moghadamnia, A.A. & Kiamahalleh, M.V. 2016. High performance curcum in subcritical water extraction from turmeric (Curcuma longa L.). Journal of Chromatography B, 1022: 191-198. CR - 8. Kwang, H.C., Lee, H.J., Koo, S.Y., Song, D.G., Lee D.U. & Pan C.H. 2010. Optimization of pressurized liquid extraction of carotenoids and chlorophylls from Chlorella vulgaris. Journal of Agricultural and Food Chemistry, 58: 793-797. CR - 9. Lai, J., Wang, H., Wang, D., Fang, F., Wang F. & Wu T. 2014. Ultrasonic extraction of antioxidants from Chinese sumac (Rhus typhina L.) fruit using RSM and their characterization. Molecules, 19: 9019-9032. CR - 10. Liang, H., Wang, W., Xu, J., Zhang, Q., Shen, Z., Zeng, Z. & Li, Q. 2017. Optimization of ionic liquid-based microwave-assisted extraction technique for curcuminoids from Curcuma longa L. Food and Bioproducts Processing, 104: 57-65. CR - 11. Lima, C.F., Pereira-Wilson, C. & Rattan, S.I. 2011. Curcumin induces hemeoxygenase-1 in normal human skin fibroblasts through redox signaling: relevance for anti-aging intervention. Molecular Nutrition & Food Research, 55: 430-442. CR - 12. Mandal, V., Mohan, Y. & S. Hemalatha. 2008. Microwave assisted extraction of curcumin by sample-solvent dual heating mechanis musing Taguchi L9 orthogonal design. Journal of Pharmaceutical and Biomedical Analysis, 46: 322-327. CR - 13. Mandal, V., Dewanjee, S., Sahu, R. & Mandal, S.C. 2009. Design and optimization of ultrasound assistedex traction of curcumin as an effective alternative for conventional solid liquid extraction of natural products. Natural Product Communications, 4: 95-100. CR - 14. Martínez-Morúa, A., Soto-Urquieta, M.G., Franco-Robles, E., Zúñiga-Trujillo, I., Campos-Cervantes, A., Pérez-Vázquez, V. & Ramírez-Emiliano, J. 2013. Curcumin decreases oxidative stress in mitochondria isolated from liver and kidneys of high-fatdiet-induced obesemice. Journal of Asian Natural Products Research, 15: 905-915. CR - 15. Matshediso, P.G., Cukrowska, E. & Chimuka, L. 2015. Development of pressurized hot water extraction (PHWE) for essential compounds from Moringa oleifera leaf extracts. Food Chemistry, 172: 423:427. CR - 16. Mourtas, S., Lazar, A.N., Markoutsa, E., Duyckaerts, C. & Antimisiaris, S.G. 2014. Multifunctional nanoliposomes with curcumin-lipid derivative and brain targeting functionality with potential applications for Alzheimer disease. European Journal of Medicinal Chemistry, 80: 175-183. CR - 17. Nasır, N., Şahin, S., Çakmak, Z. E. & Çakmak, T. 2017. Optimization of ultrasonic-assisted extraction via multiresponse surface for high antioxidant recovery from Chlorella sp. (Chlorophyta). Phycologia, 56(5): 561-569. CR - 18. Péret-Almeida, L., Cherubino, A.P.F., Alves, R.J., Dufosse, L. & Glória, M.B.A. 2005. Separation and determination of the physico-chemical characteristics of curcumin, emethoxy curcumin and bisdemethoxy curcumin. Food Research International, 38: 1039-1044. CR - 19. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. & Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radicalcation decolorization assay. Free Radical Biology and Medicine, 26: 1231-1237. CR - 20. Riela, S., Massaro, M., Colletti, C.G., Bommarito, A., Giordano, C., Milioto, S., Noto, R., Poma, P. & Lazzara, G. 2014. Development and characterization of co-loaded curcumin/triazole-halloy site systems and evaluation of their potential anticancer activity. International Immunopharmacology, 475: 613-623. CR - 21. Singleton, V.L., Orthofer, R. & Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178. CR - 22. Şahin, S., Aybastıer, Ö. & Işık, E., 2013. Optimisation of ultrasonic-assisted extraction of antioxidant compounds from Artemisia absinthium using response surface methodology. Food Chemistry, 141: 1361-1368. CR - 23. Thoo, Y.Y., Ho, S.K., Liang, J.Y., Ho, C.W. & Tan, C.P., 2010. Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chemistry, 120: 290-295. CR - 24. Xu, H.Y. & Bao, Y.H. 2014. Response surface optimization of extraction and antioxidant activity of total flavonoids from seed shell of Juglans mandshurica. Food of Science Technology Research, 20: 715-724. CR - 25. Xu, J., Wang, W., Liang, H., Zhang, Q. & Li, Q. 2015. Optimization of ionic liquid based ultrasonic assisted extraction of antioxidant compounds from Curcuma longa L. using response surface methodology. Industrial Crops and Products, 76: 487-493. CR - 26. Xu, G., Hao, C., Tian, S., Gao, F., Sun, W. & Sun, R. 2017. A method for the preparation of curcumin by ultrasonic-assisted ammonium sulfate/ethanol aqueous two phase extraction. Journal of Chromatography B, 1041-1042: 167-174. CR - 27. Yap, C.F., Ho, C.W., Wan Aida, W.M. & Chan, S.W. 2009. Optimization of extraction conditions of total phenolic compounds from star fruit (Averrhoa carambola L.) residues. Sains Malaysiana, 38(4): 511-520. UR - https://doi.org/10.23902/trkjnat.344985 L1 - https://dergipark.org.tr/en/download/article-file/521456 ER -