@article{article_356720, title={Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey}, journal={Sakarya University Journal of Science}, volume={22}, pages={1804–1811}, year={2018}, DOI={10.16984/saufenbilder.356720}, author={Colak, Dilsat Nigar and Inan Bektas, Kadriye and Tokgoz, Muslum and Canakcı, Sabriye and Belduz, Ali Osman}, keywords={Birchwood xylan,Glucose isomerase,Thermophilic bacteria}, abstract={<p class="MsoNormal" style="margin-top:0cm;margin-right:21.2pt;margin-bottom:.0001pt;margin-left:21.3pt;line-height:normal;"> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">The
aim of this study was screening of xylanase and glucose isomerase producing
thermophilic bacteria isolated from some hot springs located to Aegean Region
of Turkey. Total sixty eight thermophilic isolates ( </span> <i> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">Anoxybacillus, Brevibacillus,
Geobacillus, Aneurinibacillus, Thermus, </span> </i> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us"> <i>Paenibacillus </i>and <i> Proteobacter </i>) </span> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us"> were
collected previously from these fields and identified based on 16S rDNA gene
sequences. The isolates were screened with plate assay for determining the
xylanase and glucose isomerase production abilities seperately in order to find
new strains for industrial processes. After an incubation period of two days
for xylanase and 5-6 days for glucose isomerase at 50-60 °C, positive strains
were determined. Enzyme producing strains were confirmed by spectrophotemetric
measurements with crude enzyme extracts, birchwood xylan and glucose were used
as substrates. Most of the strains (59 strains) were positive for xylan
degradation while only sixteen of the strains had showed glucose isomerase
activity. Fourteen of the strains have showed both xylanase and glucose
isomerase activity. None of the </span> <i> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">Paenibacillus, Aneurinibacillus, </span> </i> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">and <i> Proteobacter </i> </span> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us"> strains were glucose isomerase
positive, although the glucose isomerase activity of <i>Geobacillus </i> strains were notably high. Both xylanase and glucose
isomerase activities have observed at 50-60 °C which is suitable for
biotechnological applications. </span> </p> <p> </p>}, number={6}, publisher={Sakarya University}