@article{article_363347, title={Neuroprotective Effects of Rutin and Quercetin Flavonoids in Glaucium corniculatum Methanol and Water Extracts}, journal={International Journal of Secondary Metabolite}, volume={4}, pages={85–93}, year={2017}, DOI={10.21448/ijsm.363347}, author={Koçancı, Fatma Gonca and Aslim, Belma}, keywords={Glaucium corniculatım, flavonoid, neurodegenerative diseases, anti-inflammation}, abstract={<p style="text-align:justify;"> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Neurodegenerative
diseases (NDs) are characterized by loss of neurons. NDs are associated with development
of inflammation. Existing drugs against NDs only delay the progression; however,
they do not provide a cure. The studies for the treatment of NDs focused on to discover
natural products that have the potential of anti-inhibition and anti-radical properties.
The <i>Papaveraceae </i> family members are important
for the synthesis of pharmaceutically compounds such as flavonoids which act like
anti-inflammatory drugs. In this study, methanol and water extracts of <i>Glaucium corniculatum </i>, a member of the <i>Papaveraceae </i> family, were analysed for flavonoid
compounds. The effects of extracts on neuronal PC12 cells viability was determined.
The anti-inflammatory effects of extracts were assessed by measuring the levels
of IL-6 and IL-10 cytokines on hydrogen peroxide (H <sub>2 </sub>O <sub>2 </sub>)-stimulated
PC12 cells. As a result of our studies, Rutin and Quercetin flavonoids have been
found to be as major. The amount of Rutin was higher in methanol (45 μg/ml) than
water (41 μg/ml). Quercetin was better extracted with methanol (12 μg/ml) than water
(10 μg/ml). None of the tested extracts were cytotoxic even to PC12 cells. Both
extracts showed an anti-inflammatory effect in a dose dependent manner. The water
extract showed the maximum anti-inflammatory effect, with IL-6 secretion decreased
79 fold according to the H <sub>2 </sub>O <sub>2 </sub>treated group and IL-10 secretion
increased to 87 fold according to the control group. This study is an evidence that
the Rutin and Quercetin flavonoids detected in <i>G. corniculatum </i> methanol and water extracts have a neuroprotective effect
through anti-inflammation. </span> <br /> </p>}, number={3, Special Issue 1}, publisher={İzzet KARA}