TY  - JOUR
TT  - Effect of Ammonium Nitrate Levels on Shoot and Root Formation in Micropropagation of  Apple Genotypes
AU  - Dumanoğlu, Hatice
AU  - Aygün, Ahmet
AU  - Erdoğan, Veli
PY  - 2009
DA  - February
JF  - Reserach Journal of Agricultural Sciences
JO  - Tarım Bilim. Araşt. Derg. (online)
PB  - Anatolia Bilim Akademisi
WT  - DergiPark
SN  - 1308-027X
SP  - 177
EP  - 182
IS  - 1
KW  - Anahtar kelimeler: Elma
KW  - in vitro
KW  - amonyum nitrat
KW  - sürgün oluşumu
KW  - köklenme
N2  - In this study, effect of ammonium nitrate at 1/4, 1/3, 1/2 and 1/1 strength within Murashige Skoog basal medium on shoot and root formation of ‘Golden Delicious’ and ‘Mondial Gala’ apples Malus x (domestica Borkh.) was investigated for propagation by shoot tip culture. MS media was supplemented with 1.0mg/l BA and 0.5 mg/l GA3 at shoot multiplication stage and with 0.2 mg/l IBA+0.2 mg/l NAA at rooting stage. In addition, to improve in vitro rooting, micro cuttings of difficult-to-root cultivar ‘Golden Delicious’ and easy-to-root rootstock ‘MM106’ (control) were either directly planted to MS media including 1/2 strength of macro elements plus IBA (0.25, 0.50, 1,0, 1.5, 2.0 and 3.0 mg/l) or IBA+NAA (0.5+0.5 mg/l), or they were planted to the media after fast dipping in 1000 and 4000 ppm IBA. Cultures were kept in dark during the first week of rooting experiments. The highest number of shoots was obtained in ‘Mondial Gala’ (8.7) at 1/4 strength of NH4NO3. In ‘Golden Delicious’, number of shoots per explant varied between 4.0 and 5.2, and the effect of NH4NO3 strengths on shoot number was similar statistically. Rooting was increased to 50% (Golden Delicious) and to 100% (MM106) by 1000 ppm IBA treatment in difficult-to root and easy-to root apple genotypes
UR  - https://dergipark.org.tr/en/pub/tabad/article/384607
L1  - https://dergipark.org.tr/en/download/article-file/412148
ER  -