@article{article_385330, title={The Effects of Humic Substances on Physiological Properties, Shoot Regeneration and Antioxidant Enzymatic Activities in vitro Cultured Rapeseed}, journal={Reserach Journal of Agricultural Sciences}, volume={7}, pages={55–61}, year={2014}, author={Darçın, Emine Selcen and Koç, Yusuf and Tunç, Kenan}, keywords={Brassica napus ssp. oleifera L., Humic substance, Shoot regeneration}, abstract={It was investigated the effects of various concentrations of potassium humate, boron humate and iron humate which was selected the additives to basal medium and phytohormones on physiological characteristics, antioxidant enzymatic activities and shoot regeneration rapeseed in vitro. Sterilized seeds were cultured on Murashige and Skoog Basal Medium with 3% (w/v) sucrose and 0.7% (w/v) agar containing each of the individual 1 ppm, 3 ppm, 5 ppm and 7 ppm potassium humate, boron humate and iron humate. Physiological characteristics and antioxidant enzymatic activities were observed from one week old seedlings obtained from the seeds. For observing shoot regeneration capacity, stem segments 3-4 mm in length were excised from 3-4 week old seedlings obtained from the seeds. Stem explants were cultured on Murashige and Skoog Basal Medium with 1 mgl-1 6-benzylaminopurine and 0,2 mgl-1 1-naphthaleneacetic acid. As a results, it was expressed that all the humate forms had positive effects on physiological parameters, but there were no effect on shoot regeneration. It was observed negative correlation between antioxidant enzymatic activities and increasing doses of all the humate forms}, number={1}, publisher={Anatolia Bilim Akademisi}