TY - JOUR TT - The Effects of Humic Substances on Physiological Properties, Shoot Regeneration and Antioxidant Enzymatic Activities in vitro Cultured Rapeseed AU - Darçın, Emine Selcen AU - Koç, Yusuf AU - Tunç, Kenan PY - 2014 DA - February JF - Reserach Journal of Agricultural Sciences JO - Tarım Bilim. Araşt. Derg. (online) PB - Anatolia Bilim Akademisi WT - DergiPark SN - 1308-027X SP - 55 EP - 61 VL - 7 IS - 1 KW - Brassica napus ssp. oleifera L. KW - Humik madde KW - sürgün rejenerasyonu N2 - It was investigated the effects of various concentrations of potassium humate, boron humate and iron humate which was selected the additives to basal medium and phytohormones on physiological characteristics, antioxidant enzymatic activities and shoot regeneration rapeseed in vitro. Sterilized seeds were cultured on Murashige and Skoog Basal Medium with 3% (w/v) sucrose and 0.7% (w/v) agar containing each of the individual 1 ppm, 3 ppm, 5 ppm and 7 ppm potassium humate, boron humate and iron humate. Physiological characteristics and antioxidant enzymatic activities were observed from one week old seedlings obtained from the seeds. For observing shoot regeneration capacity, stem segments 3-4 mm in length were excised from 3-4 week old seedlings obtained from the seeds. Stem explants were cultured on Murashige and Skoog Basal Medium with 1 mgl-1 6-benzylaminopurine and 0,2 mgl-1 1-naphthaleneacetic acid. As a results, it was expressed that all the humate forms had positive effects on physiological parameters, but there were no effect on shoot regeneration. It was observed negative correlation between antioxidant enzymatic activities and increasing doses of all the humate forms UR - https://dergipark.org.tr/en/pub/tabad/issue//385330 L1 - https://dergipark.org.tr/en/download/article-file/413074 ER -