TY  - JOUR
TT  - Comparison of Efficiency of Different Nucleic Acid Extraction Methods for BovineEnteroviruses in Feces
AU  - Coşkun, Nüvit
AU  - Karayel, İlke
AU  - Alkan, Feray
PY  - 2016
DA  - February
JF  - Reserach Journal of Agricultural Sciences
JO  - Tarım Bilim. Araşt. Derg. (online)
PB  - Anatolia Bilim Akademisi
WT  - DergiPark
SN  - 1308-027X
SP  - 54
EP  - 57
VL  - 9
IS  - 1
KW  - Bovine Enteroviruses
KW  - RT-PCR
KW  - Extraction
N2  - Bovine enteroviruses are thought to be mild pathogenic or non pathogenic viral gastroenteretis agents. However there are some cases where they are identified from cattle having different symptoms. They can also be used as markers of environmental contamination. Enterovirus family has fast replication capacity and can be used as potential vectors. For this purpose isolation and molecular studies concerning Enteroviruses are currently increasing. In this study 10 cattle feces samples were used for evaluation of 4 different extraction methods for investigation of bovine enteroviruses. These methods comprise of TRIzol LS (Life Technologies), QIAamp Viral RNA mini kit (Qiagen), NucleoSpin RNA (Macherey-Nagel) and phenol-chloroform extraction. All procedures performed in BSL-2 cabinet and RNA yield of extracts were analysed in Bio-spec (Shimadzu) spectrophotometer. This RNA templates were then tested by Verso 1 step RT PCR (Thermo Scientific) kit with spesific for Enterovirus 5’UTR primers. And observed for band formation. While QIAmp Viral RNA kit had the shortest protocol, it did not have as much sensitivity as TRIzol LS and TRIzol was determined as the most sensitive method to make extraction of bovine enteroviruses from feces.
UR  - https://dergipark.org.tr/en/pub/tabad/issue//385461
L1  - https://dergipark.org.tr/en/download/article-file/413230
ER  -