@article{article_386574, title={Microorganism and Pretreatment Effect on Lignocellulosic Bioethanol Production}, journal={Gazi University Journal of Science}, volume={31}, pages={1033–1046}, year={2018}, author={Bayrakcı Ozdıngıs, Asiye Gül and Kocar, Gunnur}, keywords={Clostridum thermocellum,Saccharomyces cerevisiae,Lignocellulosic ethanol,Pichia stipitis,Pretreatment}, abstract={<p> </p> <div style="text-align:justify;">The effects of pretreatments applied to raw materials and microorganism
selection in lignocellulosic bioethanol production were investigated. It has
been found that the yield of enzymatic pretreatment process applied after the
chemical pretreatment is about 4 times higher than that only chemical.
Enzymatic pretreatment used process yield is 3.5 times higher than that
chemical pretreatment. When the microorganism ethanol production yield values
of <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>Saccharomyces cerevisiae </i> </span> </font> and <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>Pichia stipitis </i> </span> </font> were examined, it was
found that <font face="Times New Roman, serif"> <span style="font-size:9pt;">S.cerevisiae </span> </font> was superior
to <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>P.stipitis </i> </span> </font> in chemical pretreated
reactors (about 1.7 times higher) while <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>P.
stipitis </i>’ </span> </font> yield was higher about 1.2 times in enzymatic pretreated
reactors. When the reactors which have been pretreated with both chemical and
enzymatic hydrolysis and <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>P. stipitis </i> </span> </font>
and  <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>S. cerevisiae </i> </span> </font> used separately were
examined, it was observed that there was not a great difference in terms of
ethanol production yield. <i> <font face="Times New Roman, serif"> <span style="font-size:9pt;">C. thermocellum </span> </font> </i>’s
ethanol yield was found about 3 times lower than the <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>S. cerevisiae </i> </span> </font> and <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>P.
stipitis </i>. </span> </font> According to the obtained data, it was seen that <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>S. cerevisiae </i> </span> </font> could produce ethanol with
higher efficiency than <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>P. stipitis </i> </span> </font>.
At the same time, the difficulty of <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>C.
thermocellum </i> </span> </font>’s production conditions, high energy demand and high risk of
contamination, and low ethanol production yield, it is thought that it can only
be used in the research phase for now. But in particular, by investigating
extracellular cellulase enzyme system of <font face="Times New Roman, serif"> <span style="font-size:9pt;"> <i>C.
thermocellum </i> </span> </font>, genetic modifications are predicted to play an important role
in the future in the second generation bioethanol production process. </div>}, number={4}, publisher={Gazi University}