TY  - JOUR
TT  - In vitro Toxicity Effects of Some Insecticides on Gilthead Sea Bream (Sparus aurata)  Liver Glucose 6-Phosphate Dehydrogenase
AU  - Kırıcı, Mahinur
AU  - Kırıcı, Muammer
AU  - Beydemir, Şükrü
AU  - Bülbül, Metin
PY  - 2016
DA  - July
JF  - Journal of Applied Biological Sciences
JO  - J.appl.biol.sci.
PB  - Anatolia Bilim Akademisi
WT  - DergiPark
SN  - 2146-0108
SP  - 46
EP  - 50
VL  - 10
IS  - 2
KW  - Glucose 6-phosphate dehydrogenase
KW  - Gilthead sea bream
KW  - Insecticides
KW  - Inhibition
N2  - In this study, effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on glucose 6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oksidoreductase, EC 1.1.1.49; G6PD) from gilthead sea bream (Sparus aurata) have been investigated in vitro. In this purpose, G6PD enzyme was purified 1864.3-fold with a yield of 54.6% from fish liver using 2’,5’-ADP Sepharose 4B affinity chromatography. Activity of the enzyme was determined by Beutler method spectrophotometrically. The purify of the enzyme was checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and the purified enzyme showed a single band on the gel. Additionally, inhibitory effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on G6PD were investigated. For this aim, IC50 values for cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were determined by plotting activity % vs. [I]. IC50 values of cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were 0.237, 0.016, 0.071, 0.021 and 0.187 mM, respectively. From these results, we showed that deltamethrin is the most potent inhibitor of fish liver G6PD enzyme
UR  - https://dergipark.org.tr/en/pub/jabs/article/387789
L1  - https://dergipark.org.tr/en/download/article-file/416207
ER  -