@article{article_387827, title={Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots}, journal={Journal of Applied Biological Sciences}, volume={11}, pages={1–4}, year={2017}, url={https://izlik.org/JA33BR86TN}, author={Özkan, Gülden ÇETİN and Khawar, Khalid Mahmood}, keywords={in vitro, mass propagation, Vicia faba, rooting, submerging}, abstract={A reliable shoot regeneration system for faba bean (Vicia faba L.) has not been reported to date to build a strong base for genetic transformation in broad bean. The present work reports a rapid, reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on MS medium containing 0.25 mg/l BAP +0.50, 0.25, 0.10, 0.05, 0.00 mg / l NAA following submerging of the seeds in distilled water for 24 hours. The explants showed a fast regeneration in 15 days time. These shoots were rooted on MS medium containing 1 mg/l IAA (15 d) and acclimatised on soil peat moss (10 days). The plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. This protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions}, number={1}