TY  - JOUR
TT  - Submerging Faba Beans in Water Improve in vitro Rooting of Microprpagated Shoots
AU  - Özkan, Gülden ÇETİN
AU  - Khawar, Khalid Mahmood
PY  - 2017
DA  - April
JF  - Journal of Applied Biological Sciences
JO  - J.appl.biol.sci.
PB  - Anatolia Bilim Akademisi
WT  - DergiPark
SN  - 2146-0108
SP  - 1
EP  - 4
VL  - 11
IS  - 1
KW  - in vitro
KW  - mass propagation
KW  - Vicia faba
KW  - rooting
KW  - submerging
N2  - A reliable shoot regeneration system for faba bean (Vicia faba L.) has not been reported to date to build a strong base for genetic transformation in broad bean. The present work reports a rapid, reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on MS medium containing 0.25 mg/l BAP +0.50, 0.25, 0.10, 0.05, 0.00 mg / l NAA following submerging of the seeds in distilled water for 24 hours. The explants showed a fast regeneration in 15 days time. These shoots were rooted on MS medium containing 1 mg/l IAA (15 d) and acclimatised on soil peat moss (10 days). The plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. This protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions
UR  - https://dergipark.org.tr/en/pub/jabs/article/387827
L1  - https://dergipark.org.tr/en/download/article-file/416244
ER  -