@article{article_391114, title={Production of Cultured Human Keratinocyte and Clinical Applications}, journal={Journal of Uludağ University Medical Faculty}, volume={30}, pages={43–46}, year={2004}, author={Özgenel, Güzin Yeşim}, keywords={Keratinocyte culture, Burn, Tissue culture, Cell transplantation}, abstract={Reconstruction of skin has been a challenging problem for surgeons in massive burns and chronic ulcerations. Autologous skin grafts are the ideal solution in reconstruction. In patients with extensive burns, surgeons often face difficulties in providing adequate permanent wound coverage because of inadequate donor autograft area. As a result of this, patients may be die of sepcis or other complications. The process in tissue engineering has led to harvest autologous epithelium by cultured keratinocytes. Although the technique of harvesting cultured epidermal autograft is expensive, grafting patients who are extensively burned with cultured epidermal autografts has been a lifesaving measure. Since cultured human keratinocytes are valuable tool especially in burn care, in this paper, the technique of harvesting cultured keratinocytes, cultured skin substitutes and their clinical use were reviewed.}, number={1}, publisher={Bursa Uludağ University}