@article{article_398669, title={Effect of Pro-Inflammatory Cytokine IL-1β, on Urotensin II Gene Expression in Human Lung Cancer Cells}, journal={Konuralp Medical Journal}, volume={10}, pages={354–359}, year={2018}, DOI={10.18521/ktd.398669}, author={Okuyan, Hamza Malik and Terzi, Menderes Yusuf and Önlen Güneri, Cansu and Urhan Küçük, Meral}, keywords={Urotensin II,IL-1β,NF-κB1,MMP-1,Lung Cancer}, abstract={ Objective: Lung cancer is the deadliest cancer type world-wide. Poor prognosis of lung cancer patients and lack of an effective treatment require detailed understanding of lung cancer pathogenesis. It was highlighted in some studies that U-II is likely to be a biomarker or molecular target for the prevention and treatment of some diseases such as lung cancer. But its molecular action mechanism has not been elucidated yet. In the present study, we aimed to investigate the role of U-II in lung cancer. Methods: In our study, A549 cells were induced with different doses of IL-1β at different durations (1, 3 ng/ml; 6, 24 hours). mRNA levels of GAPDH, NF-κB1, MMP-1, and U-II were analyzed with RT-qPCR. The Delta Delta Ct (ΔΔCt) method was used for data analysis. The analyzed data were expressed as the “fold-change”. Results: Our results indicate that U-II gene is expressed in A549 cells and IL-1β can induce gene expressions of U-II, MMP-1 and NF-κB1 in A549 cells. Conclusions: U-II is a promising molecular target in treatment and prevention of lung cancer. Therefore, further studies are needed to enlighten molecular mechanism of U-II in lung adenocarcinoma. }, number={3}, publisher={Duzce University}