TY  - JOUR
TT  - NORMAL, HİPERPLASTİK VE NEOPLASTİK ENDOMETRİYUM DOKUSUNDA CYCLIN-E VE CASPASE-3 EKSPRESYONUNUN İMMÜNOHİSTOKİMYASAL ANALİZİ
AU  - Çınar, Elif
AU  - Güngör, Tayfun
AU  - Müftüoğlu, Kamil
AU  - Özat, Mustafa
AU  - Altınkaya, Özlem
AU  - Doğanay, Melike
AU  - Tapısız, Ömer Lütfi
AU  - Bilge, Ümit
AU  - Mollamahmutoğlu, Leyla
PY  - 2009
DA  - July
JF  - Türk Jinekolojik Onkoloji Dergisi
JO  - TRSGO Dergisi
PB  - Türk Jinekolojik Onkoloji Derneği
WT  - DergiPark
SN  - 2148-5372
SP  - 60
EP  - 65
VL  - 12
IS  - 3
KW  - Cyclin-E
KW  - caspase-3
KW  - endometriyal neoplazi
N2  - Objective: Abnormalities in the control of cell proliferation and apoptosis have been suggested to contribute to the development and progression of endometrial neoplasia. The present study aimed to investigate the immunohistochemical expression of cyclin-E (cell-cycle regulatory protein) and caspase-3 (apoptosis-related protein) in normal, hyperplastic and neoplastic endometrium. Methods: Paraffin-embedded specimens from normal endometrium (n=23), simple endometrial hyperplasia without atypia (n=19) and endometrial adenocarcinoma (n=23) were stained immuno-histochemically for both intensity and distribution of cyclin-E and caspase-3. Differences in expression between the tissues were analyzed. Results: Results: Intensity of cyclin-E gradually increased from normal through hyperplasia to carcinoma (p=0.040 hyperplastic versus normal endometrium, p=0.006 hyperplastic versus neoplastic endometrium, p<0.001 normal versus neoplastic endometrium). Distribution of cyclin-E significantly increased in neoplastic endometrium compared to normal and hyperplastic endometrium (p=0.002) whereas there was no significant difference between normal and hyperplastic endometrium. No significant difference was determined in all three groups for caspase-3 intensity. Neoplastic endometrium showed significantly higher diffuse expression than normal and hyperplastic endometrium (p=0.041 and p<0.001 respectively). However, distribution of caspase-3 in normal endometrium was significantly higher than that in hyperplastic endometrium (p<0.001). Conclusions: Cyclin-E is associated with carsinogenesis and disease development and/or progression in endometrial carcinoma with regard to diffusiveness and intensity. Despite the similar expression ratios between three groups in caspase-3 intensity, higher diffuse staining in carcinomatous endometrium may indicate that caspase-3 related apoptotic pathway is also involved in the regulation of endometrial tissue and promotes the development of endometrial neoplasia.
UR  - https://dergipark.org.tr/en/pub/trsgo/issue//404501
L1  - https://dergipark.org.tr/en/download/article-file/439263
ER  -