TY  - JOUR
T1  - Oxidative stress and antioxidant defenses after starvation in Oncorhynchus mykiss (Walbaum 1792)  larvae reached free swimming stage
TT  - Serbest yüzme safhasına ulaşanOncorhynchus mykiss (Walbaum 1792)  yavrularında açlık sonrası oksidatif stres ve antioksidan savunmalar
AU  - Zengin, Hatayi
PY  - 2018
DA  - December
DO  - 10.12714/egejfas.2018.35.4.04
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege University
WT  - DergiPark
SN  - 2148-3140
SP  - 387
EP  - 396
VL  - 35
IS  - 4
LA  - en
AB  - Oxidative stress and antioxidant enzyme activities after starvation of Oncorhynchus mykiss larvae were studied. Just after yolk-sac absorption, free-swimming larvae were maintained under starved condition for a period of 21 days. Sampling of fish were carried out every week and their whole bodies were used for the analysis of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHpx), glutathione reductase (GR), glutathione S-transferase (GST) and also analysis of malondialdehyde (MDA) and glutathione (GSH). Antioxidant enzymes activities showed that GST and CAT had the highest activity, whilts SOD and GSHpx had the lowest activity of all the enzymes assayed in 14 day free swimming starved larvae. SOD activity were reached to the peak in free swimming larvae before the appearance of higher level of MDA in 7 day starved larvae. The level of MDA was significantly higher in 7 day starved larvae and significantly lower in 14 day starved larvae. ΣPUFA and Σn-3 fatty acid have the highest level in 7 day starved larvae and lowest level in 14 day starved larvae. After the free swimming larvae, there was a significant decrease in vitamin E (α-tocopherol and δ-tocopherol) level in all starved larvae.
KW  - O.mykiss larvae
KW  - oxidative stress
KW  - starvation
KW  - antioxidant enzyme
KW  - MDA & GSH
N2  - Bu çalışmada Oncorhynchus mykisslarvalarının açlıktan sonra oksidatif stres ve antioksidan enzim aktiviteleriçalışılmıştır. Yumurta kesesinin emilimiyle birlikte serbest yüzmeye geçenlarvalar 21 gün boyunca aç bırakılmıştır. Balık örnekleri her hafta alınmış vebütün vücutları, süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz(GSHpx), glutatyon redüktaz (GR), glutatyon S-transferaz (GST) analizi ve ayrıcamalondialdehit (MDA) ve glutatyon (GSH) analizinde kullanılmıştır. 14 gün açbırakılmış serbest yüzen yavrularda, analizi yapılan bütün antioksidan enzimaktiviteleri içerisinde, GST ve CAT en yüksek aktiviteye sahipken, SOD andGSHpx en düşük aktiviteye sahip olmuştur. 7 gün aç bırakılan larvalarda MDA’nınyüksek düzeyi ortaya çıkmadan önce SOD aktivitesi serbest yüzen yavrulardazirveye ulaşmıştır. MDA düzeyi 7 gün aç bırakılan larvalarda belirgin olarakdaha yüksek ve 14 gün aç bırakılan larvalarda belirgin olarak daha düşükbulunmuştur. ΣPUFA ve Σn-3 yağ asidi, 7 gün aç bırakılan larvalarda en yüksek,14 gün aç bırakılan larvalarda ise en düşük seviyeye sahip olmuştur. Serbestyüzen larvalardan sonra, aç bırakılan larvaların hepsinde E vitamini (α-tokoferolve δ- tokoferol) seviyesinde belirgin bir azalma olmuştur.
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UR  - https://doi.org/10.12714/egejfas.2018.35.4.04
L1  - https://dergipark.org.tr/en/download/article-file/608483
ER  -