TY  - JOUR
T1  - Kırmızı yanaklı su kaplumbağalarında  (Trachemys scripta elegans) Salmonella spp. varlığı ve antibakteriyel duyarlılıkları
TT  - Occurence and antibacterial susceptibility of Salmonella spp. on red-eared slider turtles (Trachemys scripta elegans)
AU  - Özer, Selmin
AU  - Konucu, Merve
AU  - Altıntaş, Hülya
AU  - Levent, Belkıs
AU  - Güleşen, Revasiye
AU  - Ülger, Mahmut
PY  - 2018
DA  - December
DO  - 10.12714/egejfas.2018.35.4.02
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege University
WT  - DergiPark
SN  - 2148-3140
SP  - 373
EP  - 380
VL  - 35
IS  - 4
LA  - tr
AB  - Son yıllarda kırmızı yanaklı sukaplumbağaları (Trachemys scripta elegans, Wied-Neuwied, 1839) ülkemizdeevcil hayvan olarak ilgi görmektedir. Sürüngen hayvanların normal bağırsakmikrobiyotasında Salmonella türlerini barındırdıkları ise bilinen bir durumdur.Ülkemizde evcil hayvan olarak satışı yapılan kırmızı yanaklı su kaplumbağalarıile ilgili yapılmış herhangi bir mikrobiyolojik araştırmaya rastlanamamıştır.Bu çalışmada, evcil hayvan işletmelerinde satışa sunulan kırmızı yanaklı sukaplumbağalarında, insanlar için patojen olan Salmonella spp. varlığı veantibakteriyel ilaç duyarlılıkları araştırılmıştır.Araştırma kapsamında, Mersin’de 10 ayrıişletmeden 10’ar adet kaplumbağanın kloaka sürüntü örneği ve kaplumbağalarıniçinde barındığı sudan 50 mL alınarak toplam 110 adet çalışılmıştır. Salmonellaizolasyonu ve identifikasyonu klasik teknikler, API20E ticari sistemi,aglütinasyon yöntemine dayalı serolojik testler ve arbitrarily primed polimerazzincir reaksiyonu (AP-PZR) uygulanarak gerçekleştirilmiştir. İzolatlarınantibakteriyel duyarlılık testi Kirby-Bauer disk difüzyon yöntemi ileyapılmıştır.Araştırma neticesinde beş adetSalmonella spp. izole edilmiş, izolatlar 100 adet kırmızı yanaklı kaplumbağakloaka sürüntü örneğinin dördünde (% 4) ve 10 adet su örneğinin birinde (% 10) saptanmıştır. İzolatların tümününSalmonella enterica subsp. enterica serovar pomona [28; y; 1,7 (z60)] ve AP-PZRsonuçlarına göre de izolatların aynı genotipik yapıya sahip olduklarıbelirlenmiştir.Antibakteriyel ilaç duyarlılık testsonuçlarına göre izolatların tamamının ampisilin, enrofloksasin, gentamisin,neomisin, siprofloksasin, streptomisin, tetrasiklin vesülfametoksazol-trimetoprime duyarlı; doksisilin’e orta derecede duyarlı;amoksisilin, eritromisin, klindamisin, linkomisin, novobiosin, penisilin vevankomisin’e dirençli oldukları anlaşılmıştır.Yapılmış olan literatür taramalarınagöre Salmonella enterica subsp. enterica serovar pomona ülkemizde ilk defasaptanmıştır. Sonuç olarak, Mersin’de satışı yapılankırmızı yanaklı su kaplumbağalarında ve paludaryum suyu örneğinde Salmonellaspp. saptanmış olup, özellikle çocuk, hamile, yaşlı ve bağışıklık sistemiyetersizliği olan bireylerin bu hayvanlarla ve suyla temaslarında hijyenkurallarına uymaları; insan sağlığının korunması amacıyla pet hayvanlardankaynaklanabilecek bulaş tehlikesinin daha ayrıntılı araştırılarak, gereklitedbirlerin alınması önem arz etmektedir.
KW  - Kırmızı yanaklı su kaplumbağası (Trachemys scripta elegans)
KW  - Salmonella spp.
KW  - AP-PZR
KW  - antibakteriyel ilaç duyarlılığı
N2  - In recent years, red-eared slider turtles (Trachemys scripta elegans, Wied-Neuwied, 1839) are popular among domestic pet animals, in Turkey. It is well known that intestinal microbiota of reptiles embody the types of human pathogenic Salmonella. In our country, there is no research about this issue. In this study the presence of Salmonella on red-eared slider turtles saled in pet shops in Mersin was determined. Also antibacterial susceptibilities of the isolates have been detected.For this purpose, a total of 100 cloacal swab samples of 100 turtles and 10 water samples, provided from ten pet shops, were studied. Isolation and identification of Salmonella have been carried out by conventional techniques, API20E trade system, agglutination tests for serological diagnosis, and arbitrarily primed PCR (AP-PCR). Antibacterial susceptibility test was performed by Kirby-Bauer disk diffusion method.As a result of this study, five Salmonella spp. have been isolated, four (4 %) from turtles, and one (10 %) from water. All the strains have been identified as Salmonella enterica subsp. enterica serovar pomona [28; y; 1.7 (z60)]. According to the results of AP-PCR the genotypic structure of the isolates were identical.The antibacterial susceptibility results of the five isolates were also identical. All isolates were sensitive to ampicillin, enrofloxacin, gentamicin, neomycin, ciprofloxacin, streptomycin, tetracycline, and sulfamethoxazole-trimethoprim; moderately sensitive to doxycycline; and resistant to amoxicillin, erythromycin, clindamycin, lincomycin, novobiocin, penicillin, and vancomycin.To best of our knowledge, this is the first detection of Salmonella enterica subsp. enterica serovar Pomona in our country. Findings of this study indicates that hygiene precautions are nessessary in contact with red eared water turtles and their paludarium water. Further investigations are necessary for protection of human health from the risk of microbiological contaminations by pet animals.
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UR  - https://doi.org/10.12714/egejfas.2018.35.4.02
L1  - https://dergipark.org.tr/en/download/article-file/608482
ER  -