TY - JOUR T1 - Erişkin İdiyopatik Trombositopenik Purpuralı Hastalarda IL-10 ve IL-17 Gen Polimorfizm TT - IL-10 and IL-17 Gene Polymorphisms in Adult Idiopathic Thrombocytopenic Purpura AU - Tat, Tuğba Songül AU - Yılmaz, Mustafa AU - Alver, Ahmet AU - Bayçelebi, Gülşah PY - 2018 DA - September JF - Namık Kemal Tıp Dergisi JO - NKMJ PB - Tekirdag Namik Kemal University WT - DergiPark SN - 2587-0262 SP - 36 EP - 44 VL - 6 IS - 2 LA - tr AB - Amaç: İnterlökin 10 (IL-10) ve interlökin 17 (IL-17) sitokingen polimorfizmlerinin otoimmün birçok hastalıkta araştırıldığı ve bazılarındaanlamlı sonuçlar bulunduğu ancak idiyopatiktrombositopenik purpuralı(ITP) hastalarda IL-10 ve IL-17 gen polimorfizmleri ile ilgili yayınlanmış az sayıda çalışma mevcut olduğu görülmüştür. Bu çalışmada erişkin ITP hastalarında IL-10 (-592 A/C) ve IL-17 (A126G) genpolimorfizmi ve bu sitokinlerin serum düzeylerinindeğerlendirilmesi amaçlanmıştır.Materyalve Metot: 50 ITP’ li hasta ve 51 sağlıklı kontrolgrubunda IL-10 (-592 A/C) ve IL-17 (A126G) gen polimorfizmleri, DNA izolasyonuyapıldıktan sonra belirlenmiş ve bu sitokinlerin serum düzeyleri ELİSA yöntemiile toplam 32 ITP’li hastada ve 32 sağlıklı kişide ölçülmüştür. Bulgular: Hastave kontrol grupları arasında IL-10 (-592 A/C) ve IL-17 (A126G) genpolimorfizmleri dağılımı açısından istatistiksel olarak anlamlı farkbulunmazken, serum IL-10 düzeyleri ITP’li hastalarda sağlıklı bireylere göreistatistiksel olarak anlamlı derecede yüksek bulunmuştur (p=0,003). Serum IL-17düzeyleri ise hasta ve kontrol grubunda benzer bulunmuştur. Ayrıca gruplararasında yaş, cinsiyet, tedavi cevabı, tanı anıdaki trombosit değerlerikarşılaştırılmış ancak gruplar arasında istatistiksel olarak anlamlı farksaptanmamıştır. Sonuç: Çalışmamızda ITP’li hastalarda sağlıklı kişilere göre IL-10 (-592A/C) ve IL-17 (A126G) gen polimorfizmleri sıklıklarının farklı olduğusaptanamasa da IL-10 düzeyleri ITP’li grupta yüksek bulunmuştur. SitokinlerinITP’nin tanı ve takibindeki önemini açıklığa kavuşturabilmek için literatürdekiveriler de dikkate alındığında bu alanda daha geniş hasta serili çalışmalaraihtiyaç olduğunu düşünmekteyiz. KW - İmmun trombositopenik purpura KW - İnterlökin-10 KW - İnterlökin -17 N2 - Objective: Interleukin 10 (IL-10) andinterleukin 17 (il-17) cytokine gene polymorphisms were investigated in manyautoimmune diseases and there were significant results in some studies butthere were a few published studies on IL-10 and il-17 gene polymorphisms in idiopathicthrombocytopenic purpura ( ITP). In this study, we aimed to evaluate the IL-10 (-592 A/C) and IL-17(A126G) gene polymorphisms and their serum levels in adult patients with ITP.Materials and Methods: The IL-10 (-592A/C) and IL-17 (A126G) gene polymorphisms in 50 patients with ITP and 51healthy control groups were determined after DNA isolation and serum levels ofthese cytokines were measured by ELISA method in 32 patients with ITP and 32healthy volunteers.Results: There was no statisticallysignificant difference in IL-10 (-592 A/C) and IL-17 (A126G) gene polymorphismsbetween the patients and control groups, whereas serum IL-10 levels were foundto be statistically significant higher in ITP patients than control group(p=0.003). Serum IL-17 levels were similar in patientsand control groups. In addition, age, sex, response to treatment and plateletvalues at diagnosis were compared between the groups, but there was nostatistically significant difference between the groups.Conclusion: In our study, IL-10 (-592A/C) and IL-17 (A126G) gene polymorphisms were not found to be different inpatients with ITP compared to healthy subjects, although IL-10 levels werefound to be higher in the ITP group than healthy group. When we look the datas in the literature, we think that morestudies with larger series are needed in this field in order to clarify theimportance of cytokines in the diagnosis and follow-up of ITP. CR - 1. Lambert MP, Gernsheimer TB. Clinical updates in adult immune thrombocytopenia. Blood. 2017; 129(21):2829–35. CR - 2. Semple J.W., Provan D. The immunopathogenesis of immune thrombocytopenia: T cells still take center-stage Curr Opinion Hematol. 2012;19:357-62 CR - 3. Raphael I, Nalawade S, Eagar TN, Forsthuber TG. 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