TY  - JOUR
T1  - Cerebrospinal Fluid Analysis of Pericytic Mediators in Clinically Isolated Syndrome and Multiple Sclerosis: A Preliminary Study
TT  - Klinik İzole Sendrom ve Multipl Skleroz Hastalarının Beyin Omurilik Sıvılarında Perisitik Mediatörlerin Analizi: Pilot Çalışma
AU  - Gündüz, Tuncay
AU  - Tanyel Kiremitçi, Tuba
AU  - Ulusoy, Canan
AU  - Kürtüncü, Murat
AU  - Türkoğlu, Recai
PY  - 2018
DA  - April
JF  - Deneysel Tıp Araştırma Enstitüsü Dergisi
PB  - Istanbul University
WT  - DergiPark
SN  - 2630-6050
SP  - 18
EP  - 22
VL  - 8
IS  - 1
LA  - en
AB  - DOI: 10.26650/experimed.2018.434227Objectives: In many studies, blood brainbarrier has been shown to be compromised in multiple sclerosis patients.Pericytes play an active role in ensuring the continuity of the blood brainbarrier along with a series of cells. In this study, the effect of pericyticdysfunction on the development of demyelinating plaques in patients withmultiple sclerosis was investigated. Material and Method: Concentrations ofpericyte dysfunction mediators (PDGFbb, MMP9, TIMP3 and ADAM17) incerebrospinal fluid of patients with clinically isolated syndrome (CIS),relapsing remitting multiple sclerosis (RRMS) and healthy control group weremeasured by ELISA and oligoclonal bands (OCB) were investigated. We aimed todetermine whether the concentration of these mediators differed between groupsand whether they correlated with lesion load, number of attacks, and EDSSscores.Results: Concentrations of all fourmediators were similar in patients with CIS and RRMS. However, both groups werefound to be higher than the healthy group. In the CIS and RRMS groups, thelevels of the mediators were not correlated with any parameters examined.However, the levels of PDGFbb (p=0.045), MMP9 (p=0.037), and TIMP3 (p=0.033)were higher in OCB positive patients than in those without OCB, whereas ADAM17levels remained unchanged.Conclusion: This study shows that pericytesmay play a role in the pathogenesis of MS from early stages of the disease. Thepresence of higher levels in patients with OCB suggests that pericytedysfunction may be associated with OCB formation.
KW  - clinically isolated syndrome
KW  - pericyte
KW  - cerebrospinal fluid
KW  - neuroinflammation
KW  - Multiple sclerosis
N2  - DOI: 10.26650/experimed.2018.434227Amaç: Multipl skleroz’da kan beyinbariyerinin bozulduğu pek çok çalışmada gösterilmiştir. Perisitler bir dizihücre yanında kan beyin bariyerinin devamlılığının sağlanmasında aktif rolüstlenir. Bu çalışmada multipl skleroz hastalarında perisit disfonksiyonunundemyelinizan plakların gelişimindeki etkisi araştırılmıştır.Gereç ve Yöntem: Klinik izole sendrom(KİS), relapsing remitting multipl skleroz (RRMS) ve sağlıklı kontrol grubundanalınan beyin omurilik sıvılarında perisit disfonksiyon mediatörlerinin (PDGFbb,MMP9, TIMP3 ve ADAM17) konsantrasyonları ELISA ile ölçüldü ve oligoklonalbantlar (OKB) araştırıldı. Bu mediyatörlerin, gruplar arasındaki farklılıklarılezyon yükü, atak sayısı ve EDSS skorları ile korelasyon gösterip göstermediğibelirlendi. Bulgular: Her dört mediyatörünkonsantrasyonları KİS ve RRMS hastalarında benzer bulundu. Bununla beraber heriki grupta sağlıklı kontollerden yüksek bulundu. KİS ve RRMS grubunda,mediyatörlerin seviyelerinin bakılan hiçbir parametre ile korelasyon göstermediğisaptandı. Buna karşın, beyin omurilik sıvılarında OKB bulunan hastalarda PDGFbb(p=0,045), MMP9 (p=0,037) ve TIMP3 (p=0,033) düzeylerinin OKB bulunmayanlaragöre daha yüksek olduğu, ADAM17 seviyelerinin ise değişmediği görüldü.Sonuç: Bu çalışma perisit disfonksiyonmediyatörlerinin MS’in patogenezinde hastalığın erken dönemlerinden itibarenrol alabileceğini göstermektedir. Oligoklonal band bulunan hastalarda dahayüksek seviyelerdeki mevcudiyeti, perisit disfonksiyonunun OKB oluşumu ile birilgisi olabileceğini akla getirmektedir.
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UR  - https://dergipark.org.tr/en/pub/iudtaed/issue//434227
L1  - https://dergipark.org.tr/en/download/article-file/533236
ER  -