TY  - JOUR
T1  - FITNESS AND PATHOGENICITY OF OUTBREAK CAUSING Salmonella UPON SHORT-TERM EXPOSURE TO GROUNDWATER WITH RESIDUAL ANTIBIOTICS
AU  - Haznedaroğlu, Berat Zeki
PY  - 2019
DA  - April
Y2  - 2018
DO  - 10.23902/trkjnat.435384
JF  - Trakya University Journal of Natural Sciences
JO  - Trakya Univ J Nat Sci
PB  - Trakya University
WT  - DergiPark
SN  - 2528-9691
SP  - 1
EP  - 8
VL  - 20
IS  - 1
LA  - en
AB  - The changes in survival andpathogenicity of three Salmonellaenterica subsp. entericaserotypes upon short term exposure to groundwater with residual antibioticshave been studied in relationship to overall microbial fitness. A wild typeflagellated Salmonella enterica ser.Typhimurium outbreak strain, a mutant Salmonellaenterica ser. Typhimurium strain, and a wild type avian disease-causing Salmonella enterica ser. Pullorum strainwere exposed to a range of ionic strength (3-30 mM) groundwater with residualantibiotics for 6-24 hours. Exposed organisms’ pathogenicity was tested invitro exposure to a human epithelial cell line (HEp2). Resistance profilesagainst 10 common antibiotics were also tested and compared to unexposedcontrols. Results show minor antibiotic resistance changes for S. enterica ser. Typhimurium strains inresponse to some antibiotic classes mediated with active efflux pumps. Thistrend was not observed for S. enterica ser.Pullorum, suggesting that resistance found in groundwater exposed organismsmight be strain-dependent. In vitro epithelial cell invasion assays showedbacterial invasion of HEp2 cells initially decreases with time and increasesafter 24 hours. It is concluded that S.enterica serotypes reaching groundwater environments in the presence ofresidual antibiotics may exhibit increased levels of pathogenicity,strain-dependent resistance to antibiotics, and sustained levels of viability.
KW  - Salmonella
KW  - antibiotic resistance
KW  - invasion
KW  - groundwater
KW  - pathogenicity
N2  - Buçalışmada kalıntı miktarda antibiyotik içeren yeraltı sularına kısa sürelimaruz bırakılan üç Salmonella entericasubsp. enterica serotipinin hastalıkyapıcı özelliklerindeki değişiklikler ve hayatta kalabilme seviyeleri genelmikrobiyel fitnes çerçevesinde incelenmiştir. Serotipler gerçek bir salgındanizole edilen kamçılı doğal fenotip Salmonellaenterica ser. Typhimurium, kamçısıfonksiyonel olmayan bir başka Salmonellaenterica ser. Typhimurium suşu vekuş türlerinde salgına yol açan doğal fenotip Salmonella enterica ser. Pullorum’dan oluşup, iyon şiddeti değişken(3-30 mM) ve kalıntı miktarda antibiyotik içeren yeraltı suyuna 6-24 saatsürecince maruz bırakılmıştır. Serotiplerin hastalık yapıcı özellikleri invitro koşullarda HEp2 türü insan epitel hücre kültürleri kullanılarakölçülmüştür. Antibiyotik direnç seviyeleri ise yaygın kullanılan 10antibitiyotiğe karşı disk difüzyon testi yardımıyla belirlenmiştir. Deneylersonucunda S. enterica ser.Typhimurium suşlarında antibiyotikdirenç seviyelerinde az miktarda artış gözlenirken S. enterica ser. Pullorum suşundadeğişiklik gözlenmemiştir. In vitro deneylerde ise suşların HEp2 hücrelerinienfekte etmeleri zamanla önce azalmış, 24 saatlik dilim sonunda ise artmıştır.Sonuç olarak, kalıtsal antibiyotik içeren yeraltı sularına temas eden S. enterica serotiplerinin hastalıkyapıcı karakterlerinin arttığı, antibiyotik direnç seviyelerinin suşa bağlıolarak değişkenlik gösterirken hayatta kalma oranlarının yüksek olduğugözlenmiştir.
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UR  - https://doi.org/10.23902/trkjnat.435384
L1  - https://dergipark.org.tr/en/download/article-file/604105
ER  -