TY - JOUR T1 - Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape AU - Gulsunoglu, Zehra AU - Kilic-akyilmaz, Meral AU - Karbancioglu-guler, Funda AU - Raes, Katleen PY - 2019 DA - December Y2 - 2018 DO - 10.15832/ankutbd.448264 JF - Journal of Agricultural Sciences JO - J Agr Sci-Tarim Bili PB - Ankara University WT - DergiPark SN - 1300-7580 SP - 459 EP - 466 VL - 25 IS - 4 LA - en AB - including cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger,Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme productionability on solid and in liquid media. Enzymatic activity was determined in the culture filtrate of liquid medium. A totalof six isolates were found to produce multiple hydrolytic enzymes. The highest activity of cellulase was produced by A.japonicus ZGM4 and A. aculeatus ZGM6 as 40 and 35 U g-1 dry biomass, respectively. All the isolates exhibited highlevel of tannase activity in the range of 150-343 U g-1 dry biomass after 24 h of incubation. A. tubingensis ZGM5 andA. aculeatus ZGM6 were found to produce the highest pectinase activity at a level of 130 and 117 U g-1 dry biomass,respectively. In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry. KW - Aspergillus KW - Cellulase KW - Pectinase KW - Tannase CR - Ang S K, Shaza E M, Adibah Y, Suraini A A, Madihah M S (2013). Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation. Process Biochemistry 48(9):1293–1302 CR - Banerjee D, Mondal K, Pati B (2007). Tannase production by Aspergillus aculeatus DBF9 through solid state fermentation. Acta Microbiologica et Immunologica Hungarica 54(2):159–166 CR - Botella C, De Ory I, Webb C, Cantero D, Blandino A (2005). Hydrolytic enzyme production by Aspergillus awamori on grape pomace. Biochemical Engineering Journal 26(2):100–106 CR - Bradoo S, Gupta R, Saxena R K (1996). Screening of extracellular tannase-producing fungi: Development of a rapid and simple plate assay. Journal of General and Applied Microbiology 42:325–329 CR - Debing J, Peijun L, Stagnitti F, Xianzhe X, Ling L (2006). Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment. Ecotoxicology and Environmental Safety 64(2):244–250 CR - Gautam S P, Bundela P S, Pandey A K, Khan J, Awasthi M K, Sarsaiya S (2011). Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnology Research International, 2011:1-8 CR - GenBank (2016). Bethesda, M D, USA: National Center for Biotechnology Information (NCBI), US National Library of Medicine. Retrieved in June, 25, 2016 from http://www.ncbi.nlm.nih.gov/ CR - Jayani R S, Saxena S, Gupta R (2005). Microbial pectinolytic enzymes: A review. Process Biochemistry 40(9):2931–2944 CR - Kar B, Banerjee R (2000). Biosynthesis of tannin acyl hydrolase from tannin-rich forest residue under different fermentation conditions. Journal of Industrial Microbiology and Biotechnology 25(1):29–38 CR - Klich M A (2002). Identification of common Aspergillus species. Utrecht, The Netherlands CR - Kuhad R C, Gupta R, Singh A (2011). Microbial cellulases and their industrial applications. Enzyme Research, doi:10.4061/2011/280696 CR - Lagemaat J V D, Pyle D L (2001). Solid-state fermentation and bioremediation: Development of a continuous process for the production of fungal tannase. Chemical Engineering Journal 84:115–123 CR - Ma W L, Zhao F F, Ye Q, Hu Z X, Yan D, Hou J, Yang Y (2015). Production and partial purification of tannase from Aspergillus ficuum Gim 3.6. Preperation of Biochemical and Biotechnology 45:754–768 CR - Mahadevan A, Sivaswamy S N (1985). Tannins and microorganisms. In: Mukerji K G (ed), Frontiers in applied microbiology, Lucknow, India: Print house, pp. 327–347 CR - Murugan K, Saravanababu S, Arunachalam M (2007). Screening of tannin acyl hydrolase (E.C.3.1.1.20) producing tannery effluent fungal isolates using simple agar plate and smf process. Bioresource Technology 98(4):946–949 CR - Raper K B, Fennell D I (1965). The genus Aspergillus. Huntington, New York CR - Schoch C L, Seifert K A, Huhndorf S, Robert V, Spouge J L, Levesque C A et al. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proceedings of the National Academy of Sciences 109(16):6241–6246 CR - Sridevi B, Charya M A S (2011). Isolation, identification and screening of potential cellulase-free xylanase producing fungi. African Journal of Biotechnology 10(22):4624–4630 CR - Taskin E, Eltem R, da Silva E S, de Souza J V B (2008). Screening of Aspergillus strains isolated from vineyards for pectinase production. Journal of Food Agriculture and Environment 6(3–4):412–414 CR - Zeni J, Cence K, Grando C E, Tiggermann L, Colet R, Lerin L A et al. (2011). Screening of pectinase-producing microorganisms with polygalacturonase activity. Applied Biochemistry Biotechnology 163(3):383–392 CR - Zheng L, Du B, Xue W (2011). Screening and identification of Acinetobacter junii for Apocynum vernetum L. fiber enzymatic retting. Journal of Textile Institute 102(8):675–680 UR - https://doi.org/10.15832/ankutbd.448264 L1 - https://dergipark.org.tr/en/download/article-file/871241 ER -