@article{article_449025, title={Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)}, journal={Kocatepe Veterinary Journal}, volume={11}, pages={430–433}, year={2018}, DOI={10.30607/kvj.449025}, author={Pekmezci, Gökmen Zafer and Pekmezci, Didem and Bölükbaş, Cenk Soner}, keywords={Demodex canis,Dog,Molecular characterization,PCR,Sequencing}, abstract={<p class="MsoNormal" style="text-align:justify;"> <span style="font-family:Garamond, serif;font-size:13.3333px;">Canine demodicosis (CD) is an inflammatory skin disease caused by excessive proliferation of  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex  </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">mites (Acarina: Demodicidae) former in the dogs’ follicles latter in the sebaceous glands. To date  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">,  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. injai </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">, and  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. cornei </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> are the three  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> species that recognized in the domestic dog  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Canis familiaris </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">. There are reports of morphological identification of  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> spp. from pet dogs in Turkey. Nevertheless there had been no reports of characterizing the  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> mite from pet dogs in Turkey using well-defined mitochondrial DNA gene sequences. The aim of the present study is to reveal first molecular characterization data on  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> infesting domestic dogs ( </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Canis familiaris </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">) in Turkey.  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> mites were obtained from skin scrapings of dogs with CD and identified microscopically as  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;">. The mitochondrial DNA 12S (mtDNA 12S) rRNA gene amplified, sequenced and compared with available  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">Demodex </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> sequences in Genbank using BLAST analyses.  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> isolate (DP-Samsun, MH374631) obtained in the study matched 100% (474/474) with previously reported gene sequences for the 12S rRNA of mtDNA in  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> (KX264486, isolate Demo.can2) from dogs in China. Pairwise comparison between the 12S rRNA sequences of the  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> from Turkey (MH374631) and other mtDNA rRNA gene of  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> isolates from China (KX264486-88) presented differences ranging from 0.0 to 0.6 %. In conclusion, within the present study, we provided the molecular characterization of  </span> <i style="font-family:Garamond, serif;font-size:13.3333px;">D. canis </i> <span style="font-family:Garamond, serif;font-size:13.3333px;"> in domestic dogs for the first time by sequencing of the partial mtDNA in Turkey.  </span> <br /> </p> <p> </p>}, number={4}, publisher={Afyon Kocatepe University}