@article{article_449087, title={Hesperidin triggering apoptosis on neuroblastoma cell}, journal={Archives of Clinical and Experimental Medicine}, volume={3}, pages={123–126}, year={2018}, DOI={10.25000/acem.449087}, author={Çatmakaş, Tolga and Ertuğrul, Barış and İplik, Elif Sinem and Çakmakoğlu, Bedia}, keywords={Hesperidin,neuroblastoma,apoptosis}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;line-height:200%;"> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Aim: </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Neuroblastoma accounts for approximately %10
of all pediatric cancers and approximately %15 of cancer related deaths in
children. Understanding of the molecular mechanisms which play role in the
progress of this cancer type may lead to develop more effective strategies for
therapy. Flavanoids are popular subject for this new strategies. Hesperidin is an
herbal flavonoid which is found abundantly in citrus that has been studied on
several cancer cell lines. For this purpose, it was aimed to investigate is the
apoptotic effects of hesperidin on neuroblastoma tumors using </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us"> </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">SH-SY5Y cell line. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;line-height:200%;"> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Methods: Hesperidin
was performed </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">on </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">SH-SY5Y and MRC-5 cell line by WST-1, Caspase-3 and
Annexin V in a time and dose dependent manner. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;line-height:200%;"> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Results: </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">2.5 µM hesperidin and 5 µM hesperidin were
found the most suitable dosage for neuroblastoma cell line because of the
success on decreasing cell proliferation. Hesperidin has resulted with the
ability for apoptotic cell death compared with control group [MRC-5 cell line,
p<0.05 for all]. 2,5 µM and 5 µM hesperidin concentration for 48h were ended
up early apoptotic results as 53.65% for 2.5 µM and 38.90% for 5 µM. There was
no significant change on caspase-3 activity. </span> </p> <p> </p> <p class="MsoNormal" style="margin:0cm 0cm .0001pt;line-height:29.333335876464844px;font-size:11pt;font-family:Cambria;"> <span lang="en-us" style="font-size:12pt;line-height:107%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Conclusions: </span> <span lang="en-us" style="font-size:12pt;line-height:107%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Our study suggests that hesperidin would be effective
against neuroblastoma tumors. We believe with further investigation this study
will be helpful for developing new research areas in neuroblastoma tumors. </span> <br /> </p> <p> </p>}, number={3}, publisher={Mustafa HASBAHÇECİ}