@article{article_455580, title={Identification of Pasteurella multocida Strains Isolated from Respiratory Tract of Healthy and Diseased Cattle and Determination of Capsular Types by PCR}, journal={Van Veterinary Journal}, volume={29}, pages={143–146}, year={2018}, author={Gulaydın, Ozgul and Gurturk, Kemal}, keywords={Pasteurella multocida,Capsular Type,PCR,Cattle}, abstract={<p align="justify"> <span lang="en-gb" style="line-height:115%;font-family:’Times New Roman’, serif;font-size:12pt;" xml:lang="en-gb">The aim of this study was to identify <i>Pasteurella </i> ( <i>P </i>.) <i>multocida </i> strains
isolated from upper and lower respiratory tract of healthy and diseased cattle
in Van region and to determine capsule type by PCR. The isolates were
identified by standard bacteriological methods and PCR (PM-PCR) using <i>KMT1 </i> gene-specific primer. From the
total of 510 examined swab samples, 60 isolates were suspected as <i>P. multocida </i> by standard bacteriological
methods and 53 (88.33%) of them were identified as <i>P. multocida </i> by PM-PCR. <i>P.
multocida </i> was isolated from 31 (13.2%) of 222 healthy cattle and 19 (32.2%)
of 59 diseased cattle. In all isolates, <i>CapA </i>
gene-specific amplicons (1044 bp) were detected by PCR using capsular type
specific primers. In conclusion, the findings of this study showed that
standard bacteriological methods were important for preliminary identification
of <i>P. multocida </i> isolates and the
isolation rate of <i>P. multocida </i> from
diseased cattle was higher than those of healthy cattle. It was also observed
that <i>P. multocida </i> capsular type A
strains were common in the respiratory tract of the cattle in Van region. </span> </p>}, number={3}, publisher={Van Yuzuncu Yıl University}