TY  - JOUR
T1  - THE EFFECT OF CALCIUM DOBESILATE ON THE PREVENTION OF EXPERIMENTAL INTRA-ABDOMINAL ADHESIONS
TT  - KALSİYUM DOBESİLAT'IN İNTRAABDOMİNAL ADEZYON ÖNLEYİCİ ETKİSİ: DENEYSEL ÇALIŞMA
AU  - Küçük, Berkay
AU  - Tuncal, Salih
AU  - Aydın, Osman
AU  - Ünal, Yılmaz
AU  - Bağ, Yusuf Murat
AU  - Adıyaman, Zeynep
AU  - Şenes, Mehmet
AU  - Celepli, Pınar
AU  - Hücümenoğlu, Sema
AU  - Kılıçoğlu, Bülent
AU  - Kısmet, Kemal
AU  - Ertaş, Ertuğrul
PY  - 2020
DA  - December
DO  - 10.20492/aeahtd.459184
JF  - Ankara Eğitim ve Araştırma Hastanesi Tıp Dergisi
PB  - Sağlık Bakanlığı Ankara Eğitim ve Araştırma Hastanesi
WT  - DergiPark
SN  - 1304-6187
SP  - 209
EP  - 214
VL  - 53
IS  - 3
LA  - en
AB  - ABSTRACT:OBJECTIVE: It was aimed to demonstrate the efficacyof Calcium Dobesilate (Ca-Dob) in preventing postoperative intra-abdominaladhesion formation in an experimental cecal abrasion model.MATERIALS AND METHODS: Thirty femaleWistar Albino rats were randomized into 3 groups. In the sham group, the cecumwas removed from the abdomen and replaced without any further processing. Inthe control group, after cecal abrasion and peritoneal resection no processingwas applied. In the Ca-Dob group, after cecal abrasion and peritoneal resection,100 mg/kg/day Ca-Dob was administered. All rats were sacrificed on 21st day.The evaluation of adhesions was made through the degrees of bowel inflammationand fibrosis in tissue samples and the biochemical analysis of tissue and bloodsamples.RESULTS: Statistically, inflammation, microscopic adhesions and fibrosis scoreswere found to be significantly lower in the Ca-Dob administered rats compared to the control group (p< 0.05). Tissue MDA, FOP and TOS values were found to be significantly lowerstatistically compared to the control group (p = 0.001). Tissue total SH valueswere found to be statistically significantly higher (p < 0.05) in the shamgroup compared with the control group. Finally; serum MDA and serum TOS values inthe Ca-Dob group, was statistically significantly lower (p < 0.05) comparedto control group.CONCLUSION: Ca-Dob was found to be efficacious in preventing theformation of adhesions. In addition to the demonstrated anti-inflammatory effecthistopathologically, the effect of antioxidant effect demonstratedbiochemically was concluded to participate to the adhesion preventive efficacyof Ca-Dob.
KW  - Calcium Dobesilate
KW  - Anti-inflammatory
KW  - Antioxidant
KW  - Adhesion
KW  - Experimental
N2  - ÖZET:AMAÇ: KalsiyumDobesilat'ın(Ca-Dob) postoperatif intraabdominal adezyon oluşumunu önlemedeki etkinliğinideneysel çekal abrazyon modelinde göstermeyi amaçladık.GEREÇ VE YÖNTEMLER: Çalışmada kullanılan 30 adet Wistar Albinodişi rat randomize olarak 3 grubaayrıldı. Sham grubuna laparotomi yapıldı, çekum batın dışına alındıktan sonraherhangi bir işlem yapılmadan batına konuldu. Kontrol grubuna çekal abrazyon veperiton rezeksiyonu sonrası herhangi bir işlem yapılmadı. Ca-Dob grubuna çekal abrazyon ve periton rezeksiyonu yapıldıktansonra batın kapatıldı, sadece bu gruba 21 gün boyunca 100 mg/kg/gün dozundaorogastrik yolla Ca-Dob verildi. Tüm ratlarpostoperatif 21. gün sakrifiye edildi. Adezyon değerlendirmesi yapıldı. Peritonve barsaktan alınan doku örnekleri inflamasyon ve fibrozis derecelerinibelirlemek için histopatolojik incelemeye gönderildi. Ayrıca, biyokimyasalanaliz için doku ve kan örnekleri alındı.BULGULAR: Ca-Dob uygulanan sıçanlardainflamasyon, makroskopik adezyon ve fibrozis skorlarının kontrol grubuna göreistatistiksel olarak anlamlı derecede düşük olduğu saptandı (p<0,05).Yine, Ca- Dob grubunda doku MDA, doku FOÜve doku TOS değerlerinin kontrol grubunagöre istatistiksel olarak anlamlı derecede düşük olduğu görüldü (p=0,001).Ayrıca sham grubundaki doku total SHdeğerlerinin, kontrol grubuna göre istatistiksel olarak anlamlı derecede yüksekolduğu saptandı (p<0,05). Son olarak; Ca- Dob grubunda serum MDA ve serum TOS değerlerinin, kontrol grubuna göre istatistiksel olarakanlamlı derecede düşük olduğu görüldü (p<0,05).SONUÇ: Ca-Dob’ınçekal abrazyon modelinde adezyon oluşumunu engellemede etkin olduğunu tespitettik. Histopatolojik olarak gösterilen antiinflamatuvar etkinin yanı sıra,biyokimyasal olarak saptanan antioksidan etkinin, Ca-Dob’ın adezyon önleyici etkinliğinde rol oynadığı sonucunavarılmıştır.
CR  - References
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UR  - https://doi.org/10.20492/aeahtd.459184
L1  - https://dergipark.org.tr/en/download/article-file/534154
ER  -