TY - JOUR T1 - Determination of Aflatoxin B1, Zearalenone and Deoxynivalenol in Feedstuffs and Aflatoxin M1 in Bulk-Tank Milk at Dairy Cattle Farms TT - Süt Sığırı İşletmelerinde Yemlerde Aflatoksin-B1, Zearalenone ve Deoxynivalenol ile Tank Sütlerinde Aflatoksin M1 Varlığının Araştırılması AU - Kara, Recep AU - Uyarlar, Cangir PY - 2018 DA - December Y2 - 2018 JF - Van Veterinary Journal JO - Van Vet J PB - Van Yuzuncu Yıl University WT - DergiPark SN - 2149-3359 SP - 179 EP - 183 VL - 29 IS - 3 LA - en AB - Mycotoxincontamination causes serious economical and toxicological problems in animalproducts. Dairy cattle breeding industry is one of the most suffering animalbreeding industry by this hazard. Generally the toxication develops chronicallyand it does not show any clinical sign on animals. Therefore it is verydifficult to identify the mycotoxin contamination in the farm site. This studyaims to determine levels of aflatoxin B1 (AF B1), total aflatoxin (B1+B2+G1+G2),zearalenone and deoxynivalenol in feedstuffs and the level of aflatoxin M1 withEnzyme-Linked Immuno Sorbent Assay (ELISA) in milk at dairy cattle companies.For this purpose, feedstuffs (corn silage, dry clover and concentrated feed)and milk (bulk tank) samples were taken from 50 different dairy cattle breedingcompanies in the Aegean Region. As aresult, levels of AF B1, total AF (B1+B2+G1+G2), zearalenone and deoxynivalenollevels were determined respectively between 0.05-36.09, 0.06-44.01, 5-675 and3-909 ppb in corn silage samples; 0.14-22.53, 0.16-27.66, 2-796 and3-692 ppb in dry clover samples; 0.12-16.90, 0.15-18.57, 4-639 and 1-877 ppb inconcentrate feed mix samples. The level of AF M1 was found between 0.001-0.054ppb in milk samples. KW - Dairy Cow KW - Aflatoxin M1 KW - Zearalenone KW - Deoxynivalenol KW - Aflatoxin B1 N2 - Mikotoksin kontaminasyonları; hayvansal ürünlerde ekonomik ve toksikolojik açıdan ciddi düzeyde problemlere neden olmaktadır. Süt sığırı yetiştiriciliği de bu zarardan etkilenen başlıca hayvancılık kolları arasında gelmektedir. Genellikle toksikasyon, kronik olarak gelişmekte ve hayvanlarda klinik olarak belirti göstermemektedir. Bu nedenle mikotoksin kontaminasyonunu çiftlik ortamında tespit etmek oldukça zordur. Bu çalışmada, süt sığırı işletmelerinde yemlerde aflatoksin B1 (AFB1), toplam aflatoksin (B1+B2+G1+G2) zearalenon ve deoksinivalenol ile tank sütlerinde aflatoksin M1 (AFM1) seviyelerinin enzyme-linked immunosorbent assay (ELISA) yöntemi ile belirlenmesi amaçlanmıştır. Bu amaçla; Ege Bölgesi’nde 50 farklı süt sığırı işletmesinden yem (mısır silajı, kuru yonca ve konsantre yem) ve tank sütü örnekleri alınmıştır. 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