TY  - JOUR
T1  - Comparison of The Expression Levels of PI3K/Akt Pathway Factors in Parkinson’s Disease and Lung Cancer
TT  - Parkinson Hastalığı ve Akciğer Kanserinde PI3K/Akt Yolağı Faktörlerinin Ekspresyon Düzeylerinin Karşılaştırılması
AU  - Şanlı, Elif
AU  - Yalçınkaya, Nazlı
AU  - Tüzün, Erdem
PY  - 2018
DA  - November
JF  - Experimed
PB  - Istanbul University
WT  - DergiPark
SN  - 2667-5846
SP  - 52
EP  - 57
VL  - 8
IS  - 2
LA  - en
AB  - DOI: 10.26650/experimed.2018.18003Objectives: Parkinson’s disease (PD) is acommon neurodegenerative disorder that affects >0.2% of the population aged>40 years. Several studies have reported a low prevalence of cancer inindividuals with PD. Therefore, the PI3K/Akt signaling pathway has receivedincreasing research attention. Investigating variations in expression levels ofcomponents associated with this pathway might aid in improving novel treatmentmethods and biomarkers for PD. Material and Method: We evaluatedexpression levels of components of the DJ-1-PI3K/Akt signaling pathway in theperipheral blood mononuclear cells of patients with PD, those with lung cancer(LC), and healthy controls and attempted to identify a possible correlationbetween expression levels and clinical features of PD. Results: Levels of phosphatase and tensinhomolog (PTEN) and apoptosis-inducing factor-1 significantly decreased, whereasthose of insulin-like growth factor 1 (IGF-1) significantly increased inpatients with PD than in healthy controls. In patients with LC, levels of PTEN,caspase-3, and α-synuclein significantly increased, whereas those of IGF-1significantly decreased. Furthermore, a correlation was observed between theclinical subtypes of PD and levels of α-synuclein, IGF-1, caspase-3, DJ-1,mechanistic target of rapamycin, Mdm2, and Akt-1 factors. Conclusion: Patients with PD showeddecreased expression levels of proapoptotic PTEN and increased levels ofcomponents of the antiapoptotic PI3K/Akt pathway, which suggests an activationof the antiapoptotic pathway to compensate for the increased neuronaldestruction in PD. The correlation between expression levels of components ofseveral PI3K/Akt signaling pathway and clinical parameters suggests that thesefactors can be used as disability and prognostic markers in patients with PD.Decreased expression levels of antiapoptotic factors in patients with PD havingpostural instability, in particular, suggests a close relationship betweenneuronal destruction and this symptom.
KW  - Parkinson’s Disease
KW  - apoptosis
KW  - PTEN
KW  - alpha-synuclein
KW  - PI3K/Akt
N2  - DOI: 10.26650/experimed.2018.18003Amaç: Parkinson hastalığı (PH) kırk yaşüzeri popülasyonun %0,2’ni etkileyen en sık görülen nörodejeneratif hastalıklardanbiridir. Yapılan çalışmalarla PH’de kanser prevalansının düşük olduğugösterilmiştir. Bu sonuçlar sebebiyle fosfoinositidil-3-kinaz (PI3K)/Aktsağkalım yolağı dikkatleri çekmiştir. Bu yolağa ait gen ekspresyondeğişikliklerinin incelenmesi, PH tedavi ve biyobelirteçlerinin bulunmasıamaçlanmıştır. Gereç ve Yöntem: Çalışmamızda, PH, akciğerkanseri (AK) hastalarından ve sağlıklı kontrollerden alınan periferik kanmononükleer hücrelerinde DJ-1-PI3K/Akt sinyal yolağı komponentlerinin ve buyolağı etkileyen proteinlerin ekspresyonu ölçülmüş, ekspresyon düzeylerininPH’nin klinik parametreleri ile korelasyonu araştırılmıştır. Bulgular: Fosfataz ve tensin homolog (PTEN)ve mitokondriyal apoptoz-indükleyici faktör 1 (Aifm1)’in PH’de sağlıklıkontrollere göre anlamlı derecede azaldığı ve insülin-benzeri büyüme faktörü(IGF-1)’in anlamlı derecede arttığı saptandı. AK hastalarında ise PTEN,kaspaz-3 ve α-sinükleinin ekspresyonlarının anlamlı derece arttığı, IGF-1’inanlamlı derecede azaldığı saptandı. Ayrıca α-sinüklein, IGF-1, kaspaz-3, DJ-1,memeli hedef rapamisin (mTOR), Mdm2 ve Akt-1 faktörleriyle PH’nin klinik alttipleri arasında korelasyon belirlendi.Sonuç: Pro-apoptotik PTEN ve anti-apoptotikPI3K/Akt yolağının ekspresyon seviyelerinde görülen değişiklik PH’de artannöronal yıkımı kompanse etmek amacıyla anti-apoptotik yolaklarınınetkinleştirildiğini düşündürmektedir. Çeşitli PI3K/Akt yolağı ekspresyondüzeyleri ile klinik parametreler arasında korelasyon saptanması PH olgularındabu faktörlerin özürlülük ve prognoz belirteci olarak kullanılabileceğinigöstermektedir. Özellikle postural instabilite ile anti-apoptotik faktörarasındaki ekspresyon değişikliği nöronal yıkımı akla getirmektedir.
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UR  - https://dergipark.org.tr/en/pub/experimed/article/468974
L1  - https://dergipark.org.tr/en/download/article-file/551157
ER  -