@article{article_469049, title={Storage Conditions for Blood Samples, for Later Analysis in Criminal Prosecution}, journal={Journal of Immunology and Clinical Microbiology}, volume={3}, pages={60–67}, year={2018}, author={Erdogan, Serpil and Erel, Ozcan and Sen, Orhan and Avcioglu, Gamze and Bal, Ceylan and Baran, Pervin}, keywords={Ethanol,stability,forensic medicine}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;"> <b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us">Backround: </span> </b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us"> Aim
of this study was to demonstrate the stability of blood–ethanol concentration
over a storage period. The precise storage time during which ethanol can be
reliably measured in frozen blood samples is unknown.  </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;"> <b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us">Materials and Methods: </span> </b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us"> In study A, samples were thawed and reanalyzed for ethanol
content; their storage time since the first analysis ranged from one to 295
days. The percent difference between the first and second ethanol measurements
was calculated as lost ethanol (%). For study B, the ethanol concentrations of
34 samples were measured within 180 days of, and at a randomly selected
interval from, the working day.  </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;"> <b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us">Results: </span> </b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us"> In
study A, the calculated lost ethanol values ranged from 1% to 30%. There was no
correlation between the calculated ethanol values and the measures on the
second ethanol analysis day. A significant difference was detected between the
two analyses in study B (p: 0.01). </span> </p> <p> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;"> <b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us">Conclusions: </span> </b> <span lang="en-us" style="font-size:10pt;line-height:115%;font-family:Cambria, serif;" xml:lang="en-us"> It
was concluded that blood samples to be analyzed for ethanol concentration can
be stored at –80°C for many months, with no loss of concentration provided that
analysis is undertaken as promptly as possible and blood samples are frozen as
soon after collection as possible. </span> </p> <p> </p> <p class="MsoNormal" style="text-align:justify;line-height:normal;"> <span lang="en-us" style="font-size:12pt;font-family:’Times New Roman’, serif;" xml:lang="en-us"> </span> </p> <p> </p>}, number={4}, publisher={Erkan YULA}