@article{article_471036, title={Effects of Nitrate toxicity on free Proline accumulation, chlorophyll degradation and photosynthetic efficiency in Chlorella vulgaris Beyerinck [Beijerinck]}, journal={International Journal of Secondary Metabolite}, volume={6}, pages={10–19}, year={2019}, DOI={10.21448/ijsm.471036}, author={Tüney Kızılkaya, İnci and Unal, Dilek}, keywords={C. vulgaris, Chlorophyll degradation, nitrate stress, proline, Fv/Fm}, abstract={<div style="text-align:justify;"> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">The
effects of high nitrate concentrations on alterations in maximal photochemical
efficiency of PSII ratio (Fv/Fm), chlorophyll content, chlorophyll degradation,
growth rate and proline accumulation in </span> <i style="font-family:’Times New Roman’, serif;font-size:10pt;">Chlorella </i> <i style="font-family:’Times New Roman’, serif;font-size:10pt;">vulgaris </i> <span style="font-family:’Times New Roman’, serif;font-size:10pt;"> </span> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">Beyerinck
[Beijerinck] </span> <b style="font-family:’Times New Roman’, serif;font-size:10pt;"> </b> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">were investigated in this study. The Fv/Fm ratio was
observed in response to the increased nitrate concentration. The Fv/Fm ratio
decreased in </span> <i style="font-family:’Times New Roman’, serif;font-size:10pt;">C. vulgaris </i> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">following 44 h in 12.99 mM NaNO </span> <sub style="font-family:’Times New Roman’, serif;">3 </sub> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">-enriched
media. Experimental results showed that, growth rates and chlorophyll content
declined by increasing nitrate concentration. The decrease in the ratio of
chlorophyll a/b with enrichment of high nitrate concentration (6.5 mM and 12.99
mM NaNO </span> <sub style="font-family:’Times New Roman’, serif;">3 </sub> <span style="font-family:’Times New Roman’, serif;font-size:10pt;">) was also caused by a decrease in chlorophyll a and an
increase in chlorophyll b concentration in </span> <i style="font-family:’Times New Roman’, serif;font-size:10pt;">C. vulgaris </i> <span style="font-family:’Times New Roman’, serif;font-size:10pt;"> cultures. The
results showed that 6.5 and 12.99 mM nitrate concentration increased proline
content in treated cells, which suggests that nitrate stress lead to proline
accumulation in </span> <i style="font-family:’Times New Roman’, serif;font-size:10pt;">C. vulgaris. </i> </div>}, number={1}, publisher={İzzet KARA}