TY  - JOUR
T1  - Investigation of Bioactive Chemicals of Passion Flower (Passiflora incarnata L.)
TT  - Çarkıfelek Çiçeğinin  (Passiflora incarnata L.) Biyoaktif Kimyasallarının İncelenmesi
AU  - Demır, Nazan
AU  - Dülger, Dilek
AU  - Ergün, Fatma
PY  - 2018
DA  - October
DO  - 10.30910/turkjans.471285
JF  - Turkish Journal of Agricultural and Natural Sciences
JO  - TURKJANS
PB  - Mevlüt AKÇURA
WT  - DergiPark
SN  - 2148-3647
SP  - 471
EP  - 476
VL  - 5
IS  - 4
LA  - en
AB  - In this study, a research wasconducted to determine the protease enzyme activity and the volatile organiccompounds of passion flower (P. incarnataL.) to determine the usability of the plant in the pharmaceutical sector. In orderto determine the activity of the protease enzyme in passion flower,measurements were taken according to the method of casein digestion in thepresence of 1% casein. In the light of the absorbance values, it is determinedthat the plant has proteolytic activity. In addition, volatile organiccompounds of the plant were analyzed by headspace GC/MSD. 5 g fresh plant wastaken and put into 20 ml headspace vial. The plant was extracted and analyzedwith 6890 GC-5975C MSD using the HP-5 MS UI column. According to the resultsobtained, it has been scientifically determined that the plant can be used inthe pharmaceutical industry.
KW  - Passion flower
KW  - Passiflora incarnata L
KW  - protease enzyme.
N2  - Bu çalışmada, çarkıfelek çiçeğinin (P. incarnata L.) proteaz enzimaktivitesi ile uçucu organic bileşenleri belirlenerek bitkinin ilaç sektöründekullanılabilirliğinin belirlenmesine yönelik bir araştırma yapılmıştır. Çarkıfelekçiçeğinde bulunan proteaz enziminin aktivitesini tayin etmek için %1’lik kazeinvarlığında kazeinin sindirimi metoduna göre ölçüm alınmıştır. Okunan absorbansdeğerleri ışığında bitkide proteolitik aktivite olduğu saptanmıştır. Ayrıcabitkinin uçucu organik bileşikleri headspace GC/MSD cihazı ile analizedilmiştir. 5 g taze bitki alınarak 20 ml headspace vialine konulmuştur. Bitkiekstraksiyon ve HP-5 MS UI kolonu kullanılarak 6890 GC-5975C MSD ile analizedilmiştir. Elde edilen sonuçlara göre bitkinin ilaç sanayinde kullanılabilirolduğu bilimsel olarak belirlenmiştir.
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UR  - https://doi.org/10.30910/turkjans.471285
L1  - https://dergipark.org.tr/en/download/article-file/555204
ER  -