TY  - JOUR
T1  - Detection of the 5HT2C polymorphism in myocardial infarction and cardiovascular event patients
TT  - Miyokard enfarktüsü ve kardiyovasküler olay geçiren hastalarda 5HT2C polimorfizminin saptanması
AU  - Açıkbaş, İbrahim
AU  - Er Urgancı, Buket
AU  - Dursunoğlu, Dursun
AU  - Kaftan, Asuman
PY  - 2019
DA  - May
Y2  - 2019
DO  - 10.31362/patd.482444
JF  - Pamukkale Medical Journal
JO  - Pam Tıp Derg
PB  - Pamukkale University
WT  - DergiPark
SN  - 1308-0865
SP  - 243
EP  - 249
VL  - 12
IS  - 2
LA  - en
AB  - Purpose:Deaths from cardiovascular (CV) disease are prevalent worldwide. Genetics,environment, nutritional habits, and lifestyleare major factors in the etiology ofCoronary artery disease (CAD). Certain genes (thatplay roles in lipid, homocysteine, glucose metabolism,renin-angiotensin, fibrinolytic, and inflammation systems) have been found inassociation with CAD by genome-wideassociation studies. The present genes are not important for direct andindependent prediction. A recent study on the rs6318 polymorphism of theserotonin receptor 2C (5HT2C) gene in the predictionof CAD and myocardial infarction (MI) can meet the expected criteria. The aim of our study is to determine the predictive and risk alleles ofthe rs6318 polymorphism of the 5HT2C gene by comparing healthy subjects withpatients diagnosed with MI and CAD. Materials and Methods:The study consisted of two groups: 142 patients and 100 controls. DNA wasisolated from venous blood and "melting curve genotyping" analysiswas performed. Results:GC genotype was 4.9% in the case group and 27% in the control group. The GCgenotype is protective against CV disease (p=0.01). In addition, theobservation that no GC genotyped subjects were diagnosed with MI suggests thatthe GC genotype is protective for MI in our study. Also, among cases with MI,it was found that only 1 patient (0.9%) was CC homozygote, while there were 12patients (11%) that were C hemizygotes. Conclusion:The results suggest that the C allele has a protective effect against CVdiseases, although no clear statistical significance is found.
KW  - 5HT2C
KW  - myocardial infarction
KW  - coronary artery disease
KW  - polymorphism
KW  - cardiovascular
N2  - Amaç:Kardiyovasküler hastalıklardan (KV) ölümler dünya genelinde ilk sırada yeralmaktadır. Etiyolojide genetik, çevre, beslenme ve yaşam tarzının etkileribulunmaktadır. Genom tarama çalışmaları, koroner arter hastalıklarıyla (KAH)ilişkili çeşitli genler (lipid, homosistein, glukoz, metabolizmaları,renin-anjiyotensin, fibnolitik, inflamasyon sistemlerinde rol alan genler)ortaya çıkarmıştır. Bunların arasında tek başına direk ve bağımsız birprediktif faktör olarak öne çıkan yok gibidir. Son yapılan bir çalışmada 5HTR2Cgenine ait rs6318 polimorfizminin, KAH ve Miyokard enfarktüs (MI) riskinibelirlemede beklenen özellikleri karşılayabileceği ortaya çıkmıştır.Çalışmamızın amacı MI ve KAH tanısı alan hastalarda 5HTR2C genindeki rs6318polimorfizmini belirlemek ve sağlıklı bireylerle karşılaştırarak koruyucu veriskli allelleri tespit etmektir.Gereç veyöntem: Çalışmamız 142 hasta ve 100 kontrol olmak üzereiki gruptan oluşmaktadır. Venöz kandan DNA izolasyonu yapıldı ve "erimeeğrisi genotiplemesi" analizi gerçekleştirildi. Bulgular:GC genotipi, olgu grubunda %4,9, kontrol grubunda %27 idi. GC genotipi, KVhastalığına karşı koruyucu olarak bulundu (p=0,01). Ek olarak, GC genotiplideneklere MI tanısı konmadığı gözlemi, çalışmamızda GC genotipinin MI içinkoruyucu olduğunu düşündürdü. Ayrıca MI'lı olgularda sadece 1 hastanın (%0,9)CC homozigot olduğu, 12'sinde (%11) ise C hemizigot olduğu saptandı.Sonuç:C allelinin belirgin bir istatistiksel anlamlılık olmamasına rağmen, KVhastalıklarına karşı koruyucu bir etkiye sahip olduğunu göstermektedir.
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UR  - https://doi.org/10.31362/patd.482444
L1  - https://dergipark.org.tr/en/download/article-file/725411
ER  -