TY  - JOUR
TT  - Identification and Prevalence of Ralstonia Solanacearum from Potato Fields of Kyrgyzstan
AU  - Döölotkeldieva, Tinatin
AU  - Bobuşeva, Saykal
PY  - 2014
DA  - June
JF  - Manas Journal of Agriculture Veterinary and Life Sciences
JO  - Manas J Agr Vet Life Sci
PB  - Kyrgyz-Turkish Manas University
WT  - DergiPark
SN  - 1694-7932
SP  - 1
EP  - 9
VL  - 4
IS  - 1
KW  - the potato (Solanum tuberosum) cultivars
KW  - identification of Ralstonia solanacearum
KW  - ELISA assay
KW  - biochemical tests
N2  - For the first time in Kyrgyzstan Raltsonia solanacearum bacterium as a pathogen of bacterial wilt (quarantine for the country object) was obtained and identified by enzyme-linked immunosorbent assay (ELISA) and biochemical methods. Three potato (Solanum tuberosum) cultivars: Picasso, Sante and Nevskiy were used for isolation of pathogen, which were collected from different regions of Kyrgyzstan. Detection and identification of the pathogen by ELISA performed directly from diseased potato shoots and leaves, and from pure culture of Ralstonia solanacearum isolated from tubers of potato seed during storage. For ELISA was used Ralstonia solanacerium PathoScreen R Kit DAS ELISA (Agdia product, USA).Isolated races of Ralstonia solanacearum by biochemical characteristics were classified as a 3-biotype.
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UR  - https://dergipark.org.tr/en/pub/mjavl/issue//484814
L1  - https://dergipark.org.tr/en/download/article-file/576725
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