TY - JOUR T1 - Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method TT - RT-PCR yöntemi kullanılarak Dejeneratif Lomber Disk Herniasyonunda İleri Düzey Protrüde ve Ekstrüde Nükleus Pulposusun Antioksidan mRNA ekspresyon düzeylerinin Karşılaştırılması AU - Yücetaş, Şeyho Cem AU - Üçler, Necati PY - 2019 DA - April Y2 - 2018 DO - 10.30569/adiyamansaglik.486946 JF - Adıyaman Üniversitesi Sağlık Bilimleri Dergisi JO - ADYÜ Sağlık Bilimleri Derg PB - Adıyaman University WT - DergiPark SN - 2458-9179 SP - 1157 EP - 1167 VL - 5 IS - 1 LA - en AB - Purpose: The aim of the present study is to compare the antioxidant levels ofnucleus pulposus in advanced protruded and extruded lumbar disc hernias (LDH)with a steady lumbar posterior longitudinal ligament with the RealTime-Polymerase Chain Reaction (RT-PCR) method.Method: Thisstudy was conducted with the comparison of the disc sections of a total of 40patients who underwent an operation due to advanced protruded (n=20) and extruded(n=20) LDH with the TRI-Reagent® and Ribonucleic acid (RNA)isolation and the RT-PCR method between January 2013 and May 2016. The studyincluded patients diagnosed with lumbar disc herniation who were applied with microdiscectomysurgery. The antioxidant levels of the disc material, which caused compressionbecause it was highly protrudedorextruded, were detected using the RT-PCRmethod,and the expression levels of the genes were determined.Results: The antioxidant levels of the disc materials of a total of 40 patients, whounderwent an operation due to protruded and extruded disc hernia, weredetermined with the RT-PCR method. The patients were found 25 females with amean age of 36.4 years and 15 males with a mean age of 39.26 years. Of the totalpatients, 20 had MacNab grade 2 (protruded) and 20 had MacNab grade 3 (extruded) disc hernias. Theexpressions of protruded LDH materials were found to be lower than those of extrudedLDH materials.Conclusion: Although theexpression levels of the significant antioxidant molecules of TGF-β, FGF-β,IGF-1, NGF, MMP-3, and GAPDH mRNA, in patients with extruded LDH weresignificantly higher compared to those with protruded LDH, no distinctivefeatures of these levels could be determined in terms of discogenic pain andpostoperative clinical improvement. KW - Lumbar microdiscectomy KW - extruded disc KW - RT-PCR KW - Gene Expression N2 - Amaç. Buçalışmanın amacı, Real Time-Polimeraz Zincir Reaksiyonu (RT-PCR) yöntemi ile lumbarposterior longitudinal ligamentte ileri düzey protrude ve ekstrüde lomber diskhernilerinde (LDH) nükleus pulposusun antioksidan düzeylerini karşılaştırmaktır.Metot: Buçalışma, Ocak 2013 ile Mayıs 2016 arasında izolasyon ve RT-PCR yöntemi ile ileridüzey protrüde (n = 20) ve ekstrüde (n = 20) LDH ile TRI-Reagent® veRibonükleik asit (RNA) ile ameliyat edilen toplam 40 hastanın disk bölümlerininkarşılaştırılması ile yapıldı. Çalışmaya, mikrodisektomi cerrahisi uygulananlomber disk herniasyonu tanısı alan hastalar dahil edildi. Sıkışmaya neden olandisk materyalinin antioksidan seviyeleri, RT-PCR yöntemi kullanılarak tespitedildi ve genlerin ekspresyon seviyeleri belirlendi.Bulgular: Protrude ve ekstrüde disk hernisi nedeniyle ameliyat edilen toplam 40hastanın disk materyallerinin antioksidan düzeyleri RT-PCR yöntemi ilebelirlendi. Çalışmadaki 25 kadın hastanın yaş ortalaması 36.4 ve 15 erkek hastanınyaş ortalaması 39.26 olduğu belirlendi. Toplamda hastaların 20'sinde MacNabgrade 2 (protruded) ve 20'sinde MacNab grade 3 (ekstrüde) disk fıtığı vardı. Ekspresyonsonucunda protrüde LDH materyallerininekstrüde LDH materyallerinden daha düşük olduğu bulundu.Sonuç: EkstrüdeLDH'si olan hastalarda TGF-β, FGF-β, IGF-1, NGF, MMP-3 ve GAPDH mRNA'nınanlamlı antioksidan moleküllerinin ekspresyon seviyeleri, protrüde LDH hastalaragöre anlamlı olarak daha yüksek bulunmasına rağmen, bu düzeylerin diskojenikağrı ve postoperative klinik iyileşmeaçısından ayırt edici özellik olarak belirlenemedi. CR - 1. Jordan J, Konstantinou K, O’Dowd J. Herniated lumbar disc. BMJ Clin Evid. 2009 Mar;2009. CR - 2. Sabnis AB, Diwan AD. The timing of surgery in lumbar disc prolapse: A systematic review. Indian J Orthop. 2014 Mar;48(2):127–35. CR - 3. Schoenfeld AJ, Weiner BK. Treatment of lumbar disc herniation: Evidence-based practice. Int J Gen Med. 2010;3:209–14. CR - 4. Soliman J, Harvey A, Howes G, Seibly J, Dossey J, Nardone E. Limited microdiscectomy for lumbar disk herniation: a retrospective long-term outcome analysis. J Spinal Disord Tech. 2014 Feb;27(1):E8–13. CR - 5. Shamim MS, Parekh MA, Bari ME, Enam SA, Khursheed F. Microdiscectomy for lumbosacral disc herniation and frequency of failed disc surgery. World Neurosurg. 2010 Dec;74(6):611–6. CR - 6. Wang D, Nasto LA, Roughley P, Leme AS, Houghton AM, Usas A, et al. Spine degeneration in a murine model of chronic human tobacco smokers. Osteoarthr Cartil. 2012;20(8):896–905. CR - 7. Battie MC, Videman T, Kaprio J, Gibbons LE, Gill K, Manninen H, et al. The Twin Spine Study: contributions to a changing view of disc degeneration. Spine J. 2009;9(1):47–59. CR - 8. Karabag H, Hatice S. The Relation of Lumbar Disc Herniation With Increased Lipid Hydroperoxide , Paraoxonase 1 and Total Oxidative Status. 2016;3(3):86–90. CR - 9. Hou G, Lu H, Chen M, Yao H, Zhao H. Oxidative stress participates in age-related changes in rat lumbar intervertebral discs. Arch Gerontol Geriatr. 2014;59(3):665–9. CR - 10. Chen JW, Ni B Bin, Li B, Yang YH, Jiang SD, Jiang LS. The responses of autophagy and apoptosis to oxidative stress in nucleus pulposus cells: Implications for disc degeneration. Cell Physiol Biochem. 2014;34(4):1175–89. CR - 11. Wang H, Song Y, Cai L. Effect of percutaneous transforaminal lumbar spine endoscopic discectomy on lumbar disc herniation and its influence on indexes of oxidative stress. Biomed Res. 2017;28(21):9464–9. CR - 12. Han Z, Wang J, Gao L, Wang Q, Wu J. Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells. Arthritis Res Ther [Internet]. 2018;20(1):182. Available from: https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-018-1677-x CR - 13. Silagi ES, Batista P, Shapiro IM, Risbud M V. Expression of Carbonic Anhydrase III, a Nucleus Pulposus Phenotypic Marker, is Hypoxia-responsive and Confers Protection from Oxidative Stress-induced Cell Death. Sci Rep [Internet]. 2018;8(1):1–13. Available from: http://dx.doi.org/10.1038/s41598-018-23196-7 CR - 14. Thermo Scientific molecular biology workflow solutions Supporting great science through innovation in molecular biology. CR - 15. Tawa N, Rhoda A, Diener I. Accuracy of magnetic resonance imaging in detecting lumbo-sacral nerve root compromise: a systematic literature review. BMC Musculoskelet Disord. 2016;17(1):386. CR - 16. Wassenaar M, van Rijn RM, van Tulder MW, Verhagen AP, van der Windt DAWM, Koes BW, et al. Magnetic resonance imaging for diagnosing lumbar spinal pathology in adult patients with low back pain or sciatica: a diagnostic systematic review. Eur spine J Off Publ Eur Spine Soc Eur Spinal Deform Soc Eur Sect Cerv Spine Res Soc. 2012 Feb;21(2):220–7. CR - 17. Wiesel SW, Weinstein JN HH et al. The Lumbar Spine. 2nd ed. Philadelphia: WB Saunders; 1996. CR - 18. Haro H, Crawford HC, Fingleton B, MacDougall JR, Shinomiya K, Spengler DM, et al. Matrix metalloproteinase-3-dependent generation of a macrophage chemoattractant in a model of herniated disc resorption. J Clin Invest. 2000 Jan;105(2):133–41. CR - 19. Haro H, Komori H, Kato T, Hara Y, Tagawa M, Shinomiya K, et al. Experimental studies on the effects of recombinant human matrix metalloproteinases on herniated disc tissues--how to facilitate the natural resorption process of herniated discs. J Orthop Res. 2005 Mar;23(2):412–9. CR - 20. Kim H-J, Park J-B, Won H-Y, Chang H. Serum Levels of TGF-β1, TIMP-1 and TIMP-2 in Patients with Lumbar Spinal Stenosis and Disc Herniation. Asian Spine J [Internet]. 2007;1(1):8. Available from: http://asianspinejournal.org/journal/view.php?doi=10.4184/asj.2007.1.1.8 CR - 21. Tolonen J, Grönblad M, Vanharanta H, Virri J, Guyer RD, Rytömaa T, et al. Growth factor expression in degenerated intervertebral disc tissue. An immunohistochemical analysis of transforming growth factor beta, fibroblast growth factor and platelet-derived growth factor. Eur spine J Off Publ Eur Spine Soc Eur Spinal Deform Soc Eur Sect Cerv Spine Res Soc. 2006 May;15(5):588–96. CR - 22. Aoki Y, Nakajima A, Ohtori S, Takahashi H, Watanabe F, Sonobe M, et al. Increase of nerve growth factor levels in the human herniated intervertebral disc: Can annular rupture trigger discogenic back pain? Arthritis Res Ther. 2014;16(4):4–5. UR - https://doi.org/10.30569/adiyamansaglik.486946 L1 - https://dergipark.org.tr/en/download/article-file/600368 ER -