@article{article_488149, title={Optimizing embryo stage and GA3 doses in Common mandarin x Carrizo citrange crosses on embryo rescue technique}, journal={Mediterranean Agricultural Sciences}, volume={32}, pages={263–266}, year={2019}, DOI={10.29136/mediterranean.488149}, author={Kurt, Senay and Ülger, Salih}, keywords={Citrus,Breeding,Pollination,Embryo rescue}, abstract={<p class="MsoNormal" style="margin:0cm -3.1pt .0001pt -2pt;" align="justify"> <span style="font-size:8pt;" lang="en-us" xml:lang="en-us"> <span style="font-size:14px;">Many difficulties are exist in citrus breeding
due to incompatibility, long juvenility, sterility and nucellar embryo. Zygotic
embryo in the seeds of polyembryonic citrus cultivar is degenerated by nucellar
embryos during embryo development. Accordingly, in vitro embryo culture is a
useful tool in citrus breeding, since it assures embryo germination and
development. In this study, </span> <span style="color:#000000;font-size:14px;">Common mandarin x Carrizo
citrange </span> <span style="font-size:14px;"> were crossed in order to produce new rootstock genotypes.
Immature embryos were taken from fruit after 80, 100 and 120 pollination days
to determine the suitable embryo rescue stage. </span> <span style="color:#000000;"> <span style="font-size:14px;"> Then, the embryos were germinated in Murashige and
Tucker (MT) culture medium including 0, 0.5 and 1 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;">.
According to the results, high germination rates were materialized as 100% and
on 95% to taken embryos from 120 days after pollination (DAP) in supplemented
with 1 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;"> and 0.5 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;">
in MT culture medium respectively. </span> </span> <span style="color:#000000;"> <span style="font-size:14px;">The embryos
taken from 80 days after pollination did not germinate on MT medium without GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;">
(control) and including with 0.5 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;">. Generally, the
existence of GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;"> on medium increased the ratio of germination
compared to the control. The highest trifoliate ratio was determined as 33.33%
at the embryos taken from 100 and 120 days after pollination and medium
including 0.5 and 1 mg l </span> <sup> <span style="font-size:14px;">-1 </span> </sup> <span style="font-size:14px;"> GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;"> consecutively. </span> </span> <span style="font-size:14px;">The
results have revealed that the optimal time for embryo rescue at citrus is 120
DAP and including GA </span> <sub> <span style="font-size:14px;">3 </span> </sub> <span style="font-size:14px;"> in the medium increased embryo germination. </span> </span> <span style="font-size:6pt;color:#000000;" lang="en-us" xml:lang="en-us"> </span> </p> <p align="justify"> <span style="font-size:14px;"> </span> <br /> </p>}, number={3}, publisher={Akdeniz University}