TY  - JOUR
T1  - The Effect of N-Acetylcysteine on Fibrosis in Experimental Rat Chronic Pancreatitis
TT  - Deneysel Kronik Pankreatitte N-Asetil Sistein Uygulamasinin Pankreatik Fibroz Üzerine Etkisi
AU  - Ocal, Ramazan
AU  - Tascı, İlker
AU  - Devecı, Mehmet Salih
AU  - Comert, Bilgin
AU  - Isık, Ahmet Turan
AU  - Mas, Mehmet Refik
PY  - 2019
DA  - March
JF  - Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi
JO  - MMJ
PB  - Mugla Sitki Kocman University
WT  - DergiPark
SN  - 2148-8118
SP  - 26
EP  - 32
VL  - 6
IS  - 1
LA  - en
AB  - In this study, weaimed to assess the effect of N-acetylcysteine on fibrosis, atrophy andoxidative stress with chronic pancreatitis in an animal model. Chronicpancreatitis is stimulated by injection of 0.4 ml, 2% trinitrobenzene sulfanicacid (TNBS) into major pancreatic ducts of the rats. 60 Sprague-Dawley ratswere randomized into four group; Group I (n=15): saline (control), Grup II(n=15): fibrosis + N-acetylcysteine (NAC), Grup III (n=15): fibrosis (TNBS),Grup IV: ethanol. At the end of the 8th week, all rats were sacrificed, and ratpancreatic tissues, oxidative stress markers and progression of fibrosis andatrophy were examined. superoxide dismutase (SOD) and glutation peroxidase(GSH-Px) activities were significantly higher with respect to TNBS group(respectively p<0.001 and p<0.001); whereas tissue malondialdehyde (MDA)levels were found significantly lower in NAC group (p<0.001). Histopathological analysis of the tissuesrevealed that histopathological score of NAC group was significantly lower thanTNBS group (p<0.003), and was found a significant relation between increasein oxidative stress and histopathological score (histopathological score/MDA:p<0.03, SOD: p<0.04, GSH-Px: p<0,02). In conclusion, administration ofNAC decreases oxidative stress in chronic pancreatitis and has beneficialeffects on fibrosis and atrophy.
KW  - Animal Model
KW  - Experimental Chronic Pancreatitis
KW  - Fibrosis
KW  - N-Acetylcysteine
N2  - Bu çalışmada,N-asetilsisteinin, kronik pankreatit hayvan modelinde fibrozis, atrofi veoksidatif stres üzerindeki etkisini değerlendirmeyi amaçladık. Kronikpankreatit, ratların ana pankreatik kanallarına 0.4 ml, %2’lik trinitrobenzensülfanik asit (TNBS) enjeksiyonu ile uyarılır. 60 Sprague-Dawley sıçanı dörtgruba randomize edildi; Grup I (n= 15): salin (sham), Grup II (n= 15): fibrozis+ N-asetilsistein (NAC), Grup III (n= 15): fibrozis (TNBS), Grup IV: etanol. 8.haftanın sonunda tüm ratlar sakrefiye edildi ve pankreatik dokular, oksidatifstres belirteçleri, fibrozis ve atrofi progresyonları incelendi. NAC grubundadoku malondialdehid (MDA) düzeyleri anlamlı olarak düşük bulunurken (p <0.001);süperoksit dismutaz (SOD) ve glutation peroksidaz (GSH-Px) aktiviteleri TNBSgrubuna göre anlamlı olarak yüksek bulundu (sırasıyla p <0.001 ve p <0.001).Dokulardaki histopatolojik incelemede NAC grubunun histopatolojik skorunun TNBSgrubundan anlamlı derecede düşük olduğu izlenirken (p<0.003) oksidatif stresile histopatolojik skordaki artış arasında anlamlı bir ilişki olduğu saptandı(histopatolojik skor/MDA: p<0.03, SOD: p<0.04, GSH-Px: p<0.02). Sonuçolarak, NAC uygulaması kronik pankreatitte oksidatif stresi azaltmakta olupfibrozis ve atrofi üzerinde yararlı etkilere sahiptir.
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UR  - https://dergipark.org.tr/en/pub/muskutd/issue//493785
L1  - https://dergipark.org.tr/en/download/article-file/674900
ER  -