@article{article_498426, title={DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY}, journal={Trakya University Journal of Natural Sciences}, volume={20}, pages={63–70}, year={2019}, DOI={10.23902/trkjnat.498426}, author={Yalçın Duygu, Dilek}, keywords={batch culture,lipid,carbohydrate,protein,ash,Nitzschia palea,ash,FTIR}, abstract={<p> <a> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb"> <font color="#000000">This study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species <i>Nitzschia </i> <i>palea </i> (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. <i> </i> </font> </span> </a> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">The study material was isolated from a fresh water sample collected from Ankara, Turkey. The diatoms were cultured in Allen medium for 168 hours and the growth dynamics were determined by cell density and dry weight analyses. Specific growth rate, duplication time of the culture and biochemical compositions were also investigated. Molecular characterization of the <i>N. palea </i> strain was performed by applying Fourier </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb"> Transform Infrared Spectroscopy. The cell density and the dry biomass of the culture at the end of the 168 hours incubation period was determined as 2.0x10 <sup>6 </sup>±1.0x10 <sup>5 </sup>cells/mL and 0.212±0.041 g L <sup>-1 </sup>, respectively. The algal specific growth rate was found as 0.010 h <sup>-1 </sup> at 96-h and the doubling time was calculated as 68 h <sup>-1 </sup>. The protein content was measured as 41.21%, carbohydrate content as </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">21.74 </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">%, lipid content as 16.84% and ash content as 19.88%. </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">These results indicated that </span> <i> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">N. palea </span> </i> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb"> </span> <span lang="en-gb" style="font-size:9pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">may be used in different fields of industries, especially in biodiesel production. </span> <br /> </p>}, number={1}, publisher={Trakya University}