@article{article_505945, title={Quantitation of Fat Soluble Vitamins in Different Walnut Genotypes and Commercial Cultivars; a Comprehensive and Robust LC-MS/MS Method Validation}, journal={Journal of the Institute of Science and Technology}, volume={9}, pages={562–571}, year={2019}, DOI={10.21597/jist.505945}, author={Yılmaz, Mustafa Abdullah}, keywords={Fat soluble vitamins,analytical method validation,LC-MS/MS,walnut}, abstract={<p class="MsoNormal" style="text-align:justify;line-height:200%;"> <span lang="en-us" style="font-size:12pt;line-height:115%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Walnut, being both nutritious and delicious, has
been regarded as a health food due to its preventive and therapeutic effects.
In the present study, a robust LC-MS/MS analytical method was developed to
simultaneously quantify five fat soluble vitamins ( <a> </a> <a> </a> <a> </a> <a>D <sub>2 </sub>, </a> D <sub>3 </sub>, K <sub>2 </sub>, E, K <sub>1 </sub>)
in eleven walnut genotypes and two commercial cultivars. Linearity, accuracy,
inter- and intra-day precision, detection limit (LOD), quantification limit
(LOQ) and uncertainty (U at confidence level of 95% (k = 2)) were the
validation parameters of the developed LC-MS/MS method. The established
linearity ranges for the studied vitamins were between <a> </a> <a> </a> <a> </a> <a> </a> <a> </a> <a>50 and
1200 mg L <sup>−1 </sup> </a> <a>for </a> <a> </a> <a> </a> <a>v </a> <a> </a> <a>itamins </a>D <sub>2 </sub>,
D <sub>3 </sub>, K <sub>1 </sub>, between 100-2400 mgL <sup>−1 </sup> for vitamin E
and between 200-4800 mg L <sup>−1 </sup> for vitamin K <sub>2 </sub>. The
coefficients of determination for the vitamins <a>D <sub>2 </sub>,
D <sub>3 </sub>, K <sub>2 </sub>, </a>E, K <sub>1 </sub> were between <a> </a> <a> </a> <a> </a> <a>0.9990 </a>,
0.9991, 0.9995, 0.9997, 0.9991, respectively. Vitamins <a>D <sub>3 </sub>, </a>
K <sub>2 </sub>and K <sub>1 </sub> were not present in the analysed walnut
samples. On the other hand, vitamins E and D <sub>2 </sub> were in high
quantities in all studied walnut samples. D <sub>2 </sub> and E were the most
abundant fat soluble vitamins in MDE32 <a> </a> <a>(29.1 mg g <sup>-1 </sup>
dry plant) </a> and MDE5 (15.2 mg g <sup>-1 </sup> dry plant) genotypes.
Despite the fact that the walnut was selected as real life sample, the
validated LC-MS/MS method is enforceable to a broad range of food species. </span> <br /> </p> <p> </p>}, number={1}, publisher={Igdir University}