@article{article_508384, title={The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells}, journal={European Journal of Biology}, volume={77}, pages={81–88}, year={2018}, author={Dagdeviren Ozsoylemez, Ozlem and Ozcan, Gul}, keywords={C-4 I,Vero,Colchicum umbrosum,paclitaxel,cancer}, abstract={<p class="MsoNormal"> <span lang="EN-US"> <span lang="EN-US" style="font-size:12.0pt;font-family: "Times",serif;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast; mso-bidi-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language: JA;mso-bidi-language:AR-SA"> <b>DOI: </b> 10.26650/EurJBiol.2018.0016 </span> <br> </span> </p> <p class="MsoNormal"> <span lang="EN-US"> <br> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Objective: This study was conducted to determine the antiproliferative effects of paclitaxel (PAC) and Colchicum umbrosum plant extract on C-4 I and Vero cells. In addition, the apoptotic effects of plant extract on these cells were compared. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Materials and Methods: C-4 I and Vero cells were treated with PAC and corm extract of C. umbrosum for 24 h. The antiproliferative effects of 0.01, 0.1, and 1 mg/mL of plant extract and 7.5, 15, and 30 nM of PAC and the half maximal inhibitory concentration (IC50) values were determined by the MTT method. Morphological changes occurring in the C-4 I and Vero cells were observed under phase-contrast and fluorescence microscopes using DAPI staining. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Results: The IC50 values were found to be 0.01 mg/mL in C-4 I cells and 1 mg/mL in Vero cells for the plant extract and 15 nM in C-4 I and Vero cells for PAC. The apoptotic index (AI) values of the experimental groups of C-4 I and Vero cells were significantly increased compared to those of the control group (p<0.01) after treatment with IC50 concentration of the plant extract. The AI values of the plant extract treatment in C-4 I cells were higher than those in Vero cells at 24 and 48 h. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Conclusion: Treatment with the IC50 concentration of the plant extract induced apoptotic cell death in C-4 I cells. It can worth be considered as a novel promising candidate for the treatment of cancer. <o:p> </o:p> </span> </p>}, number={2}, publisher={Istanbul University}