@article{article_510808, title={Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement}, journal={Konuralp Medical Journal}, volume={11}, pages={119–125}, year={2019}, DOI={10.18521/ktd.510808}, author={Geçgelen Cesur, Mine and Onal, Tuna and Bilgin, Mehmet Dincer and Sirin, Fevziye Burcu and Inan, Sevinc and Koken, Ergun Cem and Alkan, Afra and Cesur, Gokhan}, keywords={Orthodontic treatment,low intensity pulsed ultrasound,tooth movement}, abstract={<p style="margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:200%;"> <b>Objective: </b> <span lang="en-us" xml:lang="en-us">The aim of this study is to investigate the
effects of low intensity pulsed ultrasound on orthodontic tooth movement in
rats. </span> </p> <p> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:200%;"> <b> <span style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;">Methods: </span> </b> <span lang="en-gb" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-gb">For this study,
40 12-week-old adult male Wistar albino rats from the Animal Laboratory at
Adnan Menderes University, Faculty of Medicine, were used. </span> <span lang="en-us" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Rats were divided into four groups of ten. Group 1 was
the untreated control group. In group 2, an orthodontic spring was used to move
teeth. In groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16
J/cm <sup>2 </sup> or 48 J/cm <sup>2 </sup>, respectively, for 14 days. Tooth
movement was measured on day 14. Serum bone alkaline phosphatase (BALP) and
C-telopeptide of type I collagen (CTX-I) levels were analyzed biochemically.
The number of osteoclasts, osteoblasts and inflammatory cells, capillary
density and new bone formation was determined histologically. Receptor activator
of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), vascular
endothelial growth factor (VGEF) and transforming growth factor-β (TGF-β) were
assessed using immunohistochemical staining. </span> <b> <span lang="en-gb" style="font-size:12pt;line-height:200%;font-family:’Times New Roman’, serif;color:rgb(28,29,30);" xml:lang="en-gb"> </span> </b> </p> <p> <b> </b> </p> <b> </b> <p style="margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:200%;"> <b> <span lang="en-us" xml:lang="en-us">Results: </span> </b> <span lang="en-us" xml:lang="en-us">
BALP and CTX-I levels in group 4 were significantly higher than for group 1.
Tooth movement and the number of osteoclasts, inflammatory cells, and capillary
density in group 4 were significantly greater than for group 2. The intensity
levels of RANKL and OPG in group 4 were significantly greater than for group 2. </span> </p> <p> </p> <p style="margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:200%;"> <b>Conclusion: </b> <span lang="en-us" xml:lang="en-us"> Ultrasound is noninvasive application and a
promising therapy for accelerating bone remodeling during orthodontic tooth
movement. </span> </p> <p> </p>}, number={1}, publisher={Duzce University}