@article{article_514096, title={Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces}, journal={European Oral Research}, volume={52}, pages={12–18}, year={2018}, author={Yeniyol, Sinem and Ricci, John Lawrence}, keywords={Titanium dioxide,surface roughness,crystal phase,alkaline phosphatase,pre-osteoblastic cell}, abstract={<p class="MsoNormal"> <span lang="EN-US">Purpose <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">This study aimed to evaluate the initial adhesion morphology and alkaline phosphatase (ALP) activity of murine pre-osteoblastic MC3T3-E1 cells cultured on anatase/rutile mixed-phase TiO2 thin films with photocatalytical activity with previously confirmed antibacterial properties. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Materials and methods <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Anatase/rutile mixed-phase TiO2 thin films fabricated by anodization and annealing of cpTi were used to culture MC3T3-E1 cells to evaluate the initial cellular adhesion morphology and ALP activity in vitro. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Results <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Compared with MC3T3-E1 cells cultured on cpTi substrates and the control group, cells cultured on anatase/rutile mixed-phase TiO2 thin films exhibited similar ALP levels after cell culture day 9. <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Conclusion <o:p> </o:p> </span> </p> <p class="MsoNormal"> <span lang="EN-US">Anodizing and annealing processes fabricate multifunctional surfaces on cpTi with improved osteogenic properties for implants. <o:p> </o:p> </span> </p>}, number={1}, publisher={Istanbul University}