TY  - JOUR
T1  - THE EFFECT OF NITRIC OXIDE SYNTHASE ON BETA 3 ADRENOCEPTOR MEDIATED RELAXATION IN STZ DIABETIC RAT HEART
TT  - STZ DİABETİK SIÇAN KALBİNDE BETA 3 ADRENOSEPTÖR ARACILI GEVŞEME YANITINDA NİTRİK OKSİT SENTAZIN ETKİSİ
AU  - Arıoğlu İnan, Ebru
AU  - Kaykı Mutlu, Gizem
AU  - Karaömerlioğlu, İrem
PY  - 2019
DA  - January
Y2  - 2019
DO  - 10.33483/jfpau.519948
JF  - Journal of Faculty of Pharmacy of Ankara University
JO  - J. Fac. Pharm. Ankara
PB  - Ankara University
WT  - DergiPark
SN  - 1015-3918
SP  - 64
EP  - 71
VL  - 43
IS  - 1
LA  - en
AB  -         Objective: Beta 3 adrenoceptors have been shown to mediatenegative inotropic effect in the heart. In the dibetic rat heart, beta 3adrenoceptor related relaxation response has been found to be augmented. In thepresent study, we aimed to investigate the contribution of nitric oxide andnitric oxide synthase subtypes on beta 3 adrenoceptor mediated relaxationresponse in chronic STZ diabetic rat heart.        Material and Method: 8-week old sprague dawley rats wereused in the study. Diabetes was induced by single dose streptozotocin injection.Some of the diabetic rats were treated with insulin. Beta 3 adrenoceptormediated negative inotropic effect was evaluated by using CL 316,243 in theabsence and presence of L-NAME. The expression of beta 3 adrenoceptor, eNOS,phosphorylated eNOS and nNOS were determined by western blot experiments.        Result and Discussion: The relaxation response was increased in diabetic heart and notnormalized after insulin treatment. The effect was abolished after L-NAMEincubation. Beta 3 adrenoceptor was upregulated in diabetic heart and reachedto control values after treatment. Expression of eNOS, phosphorylated eNOS ornNOS was not altered significantly among the groups. The results of the studyindicate that beta 3 adrenoceptor mediated relaxation in diabetic heart isnitric oxide related.
KW  - Diabetes
KW  - heart
KW  - beta 3 adrenoceptor
KW  - nitric oxide synthase
N2  - Amaç: Beta 3 adrenoseptörlerin kalptenegatif inotropik etkiye aracılık ettiği gösterilmiştir. Diabetik sıçankalbinde, beta 3 adrenoseptör aracılı gevşeme yanıtının belirgin hale geldiğibulunmuştur. Bu çalışmada, kronik STZ diabetik sıçan kalbinde nitrik oksit venitrik oksit sentetaz alttiplerinin beta 3 adrenoseptör aracılı gevşemeyanıtına katkısının araştırılması amaçlanmıştır.        Gereç ve Yöntem: Çalışmada 8-haftalık spraguedawley sıçan kullanılmıştır. Diabet tek doz streptozotosin injeksiyonu ilegerçekleştirilmiştir. Bazı diabetik sıçanlar insulin ile tedavi edilmiştir.Beta 3 adrenoseptör aracılı gevşeme yanıtı CL 316,243 kullanılarak L-NAMEvarlığında ve yokluğunda değerlendirilmiştir. Beta 3 adrenoseptör, eNOS,fosforile eNOS ve nNOS ekspresyonu western blot yöntemi ile ölçülmüştür.        Sonuç ve Tartışma: Diabetik kalpte CL-aracılıgevşeme yanıtları artmıştır, ancak insulin tedavisi sonrasında kontrol düzeyinegelmemiştir. Gözlenen etki L-NAME inkübasyonu sonrasında ortadan kalkmıştır.Beta 3 adrenoseptör ekspresyonu diabetik kalpte artmıştır ve tedavi sonrasıkontrol seviyesine geri dönmüştür. eNOS, fosforile eNOS ve nNOS düzeylerigruplararasında anlamlı bir fark bulunamamıştır. Çalışmanın sonuçları, diabetiksıçan kalbinde beta 3 adrenoseptör aracılı gevşeme yanıtının nitrik oksitbağlantılı olduğunu ortaya koymaktadır.    
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UR  - https://doi.org/10.33483/jfpau.519948
L1  - https://dergipark.org.tr/en/download/article-file/637895
ER  -