TY - JOUR T1 - Production of Chlorella sp. in a Designed Photobioreactor AU - Köse, Merve Deniz AU - Ak, Büsra AU - Atak, Eylem AU - Bayraktar, Oğuz PY - 2019 DA - December DO - 10.18466/cbayarfbe.523332 JF - Celal Bayar University Journal of Science JO - CBUJOS PB - Manisa Celal Bayar University WT - DergiPark SN - 1305-130X SP - 377 EP - 383 VL - 15 IS - 4 LA - en AB - Click here for manuscript sample templateMicroalgae arephotosynthetic microorganisms which are recently grown to produce biomass for food, pharmaceutical, dye, and bioenergy industries. Microalgae are thecarbon source found in crude oil and natural gas. Over the years, therehave been several advances in the design and operation of closed photobioreactorsfor microalgal cultures based on new reactor geometries as well as optimized aerationand mixing strategies. Closed photobioreactors ensure heatcontrol, and high production through effective use of high-intensity light and prevent contamination in microalgaeproduction. One of the most important factors thatcontrol cell growth in a photobioreactor is light availability. In this study,cultivation of Chlorella sp., asmicroalgae were performed in a speciallydesigned photobioreactor for productivity analysis and the pigment capacityanalysis. The applied light energy was kept constant while applying eithercontinuous or intermittent lighting during the growth of microalgae. Thecultivation parameters were tested to find the optimal light mode as the continuouslight or 12h light/ 12h dark cycle to maximize pigment amount. In order todetermine the pigment amount in the cultivated algae extraction was done. Thenby using UV spectrophotometer amount of chlorophyll a and b were determined inthe obtained extracts. KW - Microalgae KW - Chlorella sp. KW - Photobioreactor design KW - Extraction KW - Pigments CR - 1. Brasier, M. D., Green, O. R., Jephcoat, A. P., Kleppe, A. K., Van Kranendonk, M. J., Lindsay, J. F., ... & Grassineau, N. V. 2002. Questioning the evidence for Earth's oldest fossils. Nature, 416(6876), 76. CR - 2. Converti, A., Casazza, A. A., Ortiz, E. Y., Perego, P., & Del Borghi, M. 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification, 48(6), 1146-1151.7 CR - 3. González‐Fernández, C., Sialve, B., Bernet, N., & Steyer, J. P. 2012. Impact of microalgae characteristics on their conversion to biofuel. Part I: Focus on cultivation and biofuel production. Biofuels, Bioproducts and Biorefining, 6(1), 105-113. CR - 4. Safi, C., Zebib, B., Merah, O., Pontalier, P. Y., & Vaca-Garcia, C. 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: a review. Renewable and Sustainable Energy Reviews, 35, 265-278. CR - 5. Lee, R., Phycology. Cambridge [England]: Cambridge University Press., 4th ed., 1999, pp.213-217. CR - 6. Abu-Ghosh, S., Fixler, D., Dubinsky, Z. and Iluz, D. 2015. Continuous background light significantly increases flashing-light enhancement of photosynthesis and growth of microalgae. Bioresource Technology, 187, 144-148. CR - 7. Carvalho, A., Silva, S., Baptista, J. and Malcata, F. 2010. Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Applied Microbiology Biotechnology, 89(5), 1275-1288. CR - 8. Yılmaz, H. K. 2006. Mikroalg Üretimi için Fotobiyoreaktör Tasarımları. EÜ Su Ürünleri Dergisi, 23(1/2), 327-332. CR - 9. Walker, T. L., Purton, S., Becker, D. K., & Collet, C. 2005. Microalgae as bioreactors. Plant cell reports, 24(11), 629-641. CR - 10. Kim, J., Lee, J. and Lu, T. 2015. A model for autotrophic growth of Chlorella vulgaris under photolimitation and photoinhibition in cylindrical photobioreactor. Biochemical Engineering Journal, 99, 55-60. CR - 11. Andersen, R. A. (Ed.). 2005. Algal culturing techniques. Elsevier. CR - 12. ATCC Medium: 616 Medium BG-11 for Blue-green Algae, www.atcc.org CR - 13. Shuler, M. and Kargi, F., Bioprocess engineering. Upper Saddle River, NJ: Prentice Hall 2002. CR - 14. Jeffrey, S.W., Humphrey, G.F. 1975. New spectrophotometric equations for determining chlorophyll a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Journal of Plant Biochemistry Physiology, 167, 191–194. CR - 15. Pottier, L., Pruvost, J., Deremetz, J., Cornet, J., Legrand, J. and Dussap, C. 2005. A fully predictive model for one-dimensional light attenuation by Chlamydomonas reinhardtii in a torus photobioreactor. Biotechnology and Bioengineering, 91(5), 569-582. UR - https://doi.org/10.18466/cbayarfbe.523332 L1 - https://dergipark.org.tr/en/download/article-file/904947 ER -